Effects of death delay time on brain tissue staining based on synchronous X-ray multimodal imaging

doi:10.11889/j.0253-3219.2022.hjs.45.040103

	Effects of death delay time on brain tissue staining based on synchronous X-ray multimodal imaging
其他题名	应用同步辐射X射线多模态成像研究死亡延迟时间对脑组织染色的影响
	Yan, Xin1,2 ; Cai, Xiaoqing 1,3,4; Tang, Qiaowei 1,4; Zhou, Feng 1,4; Zhu, Ying 1,3,4; Wang, Lihua 1,3,4; Hu, Jun1,2,3,4
	2022-04-15
发表期刊	HE JISHU/NUCLEAR TECHNIQUES
ISSN	0253-3219
卷号	45 期号:4
发表状态	已发表
DOI	10.11889/j.0253-3219.2022.hjs.45.040103
摘要	Background: Human brain tissue is usually obtained by donating, and post-mortem delay (PMD) is one of the important factors affecting Golgi staining in human brain. However, currently there are relatively few studies on how PMD affects Golgi staining in human brain tissue. Purpose: This study aims to explore the changes in the morphology and structure of pyramidal neurons in the cerebral cortex of mice with PMD. Method: The changes of human brain PMD were simulated by using mouse brain tissues placed in vitro for different times, and light microscope imaging data were obtained by Golgi staining and Nissl staining. At the same time, the synchrotron X-ray micro-CT imaging technology was used to realize neurons three-dimensional imaging visualization. The Sholl analysis was employed to study the Golgi-stained pyramidal neurons in the cerebral cortex. Results: Analysis results show that the completeness and complexity emerge a significant decreasing trend with the increase of PMD, and the statistical results of dendritic spines also show the same decrease trend. The three-dimensional neuron visualization results reconstructed by synchrotron radiation X-ray micro-CT technology confirm the above analysis results. Conclusions: This study provides a research foundation for the establishment of Golgi staining method of human brain tissue and synchrotron radiation X-ray imaging. © 2022, Science Press. All right reserved.
关键词	Brain Computerized tomography Mammals Neurons Synchrotrons Three dimensional computer graphics Tissue Visualization Brain tissue Cortical pyramidal neuron CT technology Golgi staining Human brain Neuron structure Post mortem Pyramidal neuron Synchrotron X-ray micro-CT technology X-ray micro-CT
收录类别	EI
语种	中文
出版者	Science Press
EI入藏号	20240615515789
EI主题词	Synchrotron radiation
EI分类号	461.1 Biomedical Engineering ; 461.2 Biological Materials and Tissue Engineering ; 461.9 Biology ; 723.2 Data Processing and Image Processing ; 723.5 Computer Applications ; 932.1.1 Particle Accelerators
原始文献类型	Journal article (JA)
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/359946
专题	物质科学与技术学院_硕士生物质科学与技术学院_特聘教授组_胡钧组
作者单位	1.Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai; 201800, China; 2.ShanghaiTech University, Shanghai; 201210, China; 3.The Interdisciplinary Research Center, Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai; 201210, China; 4.University of Chinese Academy of Sciences, Beijing; 100049, China
第一作者单位	上海科技大学
推荐引用方式 GB/T 7714	Yan, Xin,Cai, Xiaoqing,Tang, Qiaowei,et al. Effects of death delay time on brain tissue staining based on synchronous X-ray multimodal imaging[J]. HE JISHU/NUCLEAR TECHNIQUES,2022,45(4).
APA	Yan, Xin.,Cai, Xiaoqing.,Tang, Qiaowei.,Zhou, Feng.,Zhu, Ying.,...&Hu, Jun.(2022).Effects of death delay time on brain tissue staining based on synchronous X-ray multimodal imaging.HE JISHU/NUCLEAR TECHNIQUES,45(4).
MLA	Yan, Xin,et al."Effects of death delay time on brain tissue staining based on synchronous X-ray multimodal imaging".HE JISHU/NUCLEAR TECHNIQUES 45.4(2022).