Identification of a broad sarbecovirus neutralizing antibody targeting a conserved epitope on the receptor-binding domain

doi:10.1016/j.celrep.2023.113653

KMS > 免疫化学研究所

	Identification of a broad sarbecovirus neutralizing antibody targeting a conserved epitope on the receptor-binding domain
	Wang, Yanqun 1,2; Zhang, Zhaoyong ; Yang, Minnan 3; Xiong, Xinyi5,7 ; Yan, Qihong ; Cao, Lei 3; Wei, Peilan 5; Zhang, Yuting ; Zhang, Lu 6; Lv, Kexin ; Chen, Jiantao ; Liu, Xuesong 9; Zhao, Xiaochu 10; Xiao, Juxue ; Zhang, Shengnan ; Zhu, Airu ; Gan, Mian ; Zhang, Jingjun ; Cai, Ruoxi ; Zhuo, Jianfen ; Zhang, Yanjun ; Rao, Haiyue ; Qu, Bin 4; Zhang, Yuanyuan ; Chen, Lei ; Dai, Jun 6; Cheng, Linling ; Hu, Qingtao ; Chen, Yaoqing 7; Lv, Huibin 11; So, Ray T. Y.12; Peiris, Malik 11,12; Zhao, Jingxian ; Liu, Xiaoqing 9; Mok, Chris Ka Pun 13,14,16; Wang, Xiangxi 3; Zhao, Jincun4,5,15
	2024-01-23
发表期刊	CELL REPORTS (IF:7.5[JCR-2023],8.5[5-Year])
ISSN	2211-1247
卷号	43 期号:1
发表状态	已发表
DOI	10.1016/j.celrep.2023.113653
摘要	Omicron, as the emerging variant with enhanced vaccine tolerance, has sharply disrupted most therapeutic antibodies. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) belongs to the subgenus Sarbecovirus, members of which share high sequence similarity. Herein, we report one sarbecovirus antibody, 5817, which has broad-spectrum neutralization capacity against SARS-CoV-2 variants of concern (VOCs) and SARS-CoV, as well as related bat and pangolin viruses. 5817 can hardly compete with six classes of receptor -binding -domain -targeted antibodies grouped by structural classifications. No obvious impairment in the potency is detected against SARS-CoV-2 Omicron and subvariants. The cryoelectron microscopy (cryoEM) structure of neutralizing antibody 5817 in complex with Omicron spike reveals a highly conserved epitope, only existing at the receptor -binding domain (RBD) open state. Prophylactic and therapeutic administration of 5817 potently protects mice from SARS-CoV-2 Beta, Delta, Omicron, and SARS-CoV infection. This study reveals a highly conserved cryptic epitope targeted by a broad sarbecovirus neutralizing antibody, which would be beneficial to meet the potential threat of pre -emergent SARS-CoV-2 VOCs.
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收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2023YFC3041600] ; National Natural Science Foundation of China[
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:001154909100001
出版者	CELL PRESS
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/349917
专题	免疫化学研究所生命科学与技术学院_博士生免疫化学研究所_特聘教授组_赵金存组
通讯作者	Wang, Yanqun; Liu, Xiaoqing; Mok, Chris Ka Pun; Wang, Xiangxi; Zhao, Jincun
作者单位	1.Guangzhou Med Univ, Guangzhou Inst Resp Hlth, Natl Clin Res Ctr Resp Dis, State Key Lab Resp Dis,Affiliated Hosp 1, Guangzhou 510120, Guangdong, Peoples R China 2.Guangzhou Med Univ, GMU GIBH Joint Sch Life Sci, Guangzhou, Guangdong, Peoples R China 3.Guangzhou Med Univ, Clin Lab Med Dept, Affiliated Hosp 2, Guangzhou, Peoples R China 4.Chinese Acad Sci, Inst Biophys, CAS Key Lab Infect & Immun, Natl Lab Macromol, Beijing, Peoples R China 5.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Sch Life Sci & Technol, Shanghai, Peoples R China 6.Guangzhou Natl Lab, Guangzhou, Peoples R China 7.Guangzhou Customs Dist Technol Ctr, Hlth & Quarantine Lab, Guangzhou, Peoples R China 8.Sun Yat Sen Univ, Sch Publ Hlth Shenzhen, Shenzhen, Peoples R China 9.Guangzhou Med Univ, Guangzhou Inst Resp Hlth, Natl Clin Res Ctr Resp Dis, Dept Crit Care Med,Affiliated Hosp 1,State Key Lab, Guangzhou, Peoples R China 10.Capital Med Univ, Sch Basic Med Sci, Beijing, Peoples R China 11.Univ Hong Kong, Li Ka Shing Fac Med, Sch Publ Hlth, HKU Pasteur Res Pole, Hong Kong, Peoples R China 12.Univ Hong Kong, Sch Publ Hlth, Hong Kong, Peoples R China 13.Chinese Univ Hong Kong, Jockey Club Sch Publ Hlth & Primary Care, Hong Kong, Peoples R China 14.Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Sci, Fac Med, Hong Kong, Peoples R China 15.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Affiliated Hosp 2, Inst Hepatol,Sch Med ,Natl Clin Res Ctr Infect Dis, Shenzhen, Peoples R China 16.Chinese Univ Hong Kong, SH Ho Res Ctr Infect Dis, Hong Kong, Peoples R China
通讯作者单位	免疫化学研究所
推荐引用方式 GB/T 7714	Wang, Yanqun,Zhang, Zhaoyong,Yang, Minnan,et al. Identification of a broad sarbecovirus neutralizing antibody targeting a conserved epitope on the receptor-binding domain[J]. CELL REPORTS,2024,43(1).
APA	Wang, Yanqun.,Zhang, Zhaoyong.,Yang, Minnan.,Xiong, Xinyi.,Yan, Qihong.,...&Zhao, Jincun.(2024).Identification of a broad sarbecovirus neutralizing antibody targeting a conserved epitope on the receptor-binding domain.CELL REPORTS,43(1).
MLA	Wang, Yanqun,et al."Identification of a broad sarbecovirus neutralizing antibody targeting a conserved epitope on the receptor-binding domain".CELL REPORTS 43.1(2024).