BacPE: a versatile prime-editing platform in bacteria by inhibiting DNA exonucleases

doi:10.1038/s41467-024-45114-4

	BacPE: a versatile prime-editing platform in bacteria by inhibiting DNA exonucleases
	Zhang, Hongyuan1,2 ; Ma, Jiacheng1,2 ; Wu, Zhaowei1,2 ; Chen, Xiaoyang1,2 ; Qian, Yangyang3 ; Chen, Weizhong1,2,4 ; Wang, Zhipeng1,2 ; Zhang, Ya1,2 ; Zhu, Huanhu3 ; Huang, Xingxu5,6 ; Ji, Quanjiang1,2,6,7
	2024-01-27
发表期刊	NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year])
EISSN	2041-1723
卷号	15 期号:1
发表状态	已发表
DOI	10.1038/s41467-024-45114-4
摘要	["Prime editing allows precise installation of any single base substitution and small insertions and deletions without requiring homologous recombination or double-strand DNA breaks in eukaryotic cells. However, the applications in bacteria are hindered and the underlying mechanisms that impede efficient prime editing remain enigmatic. Here, we report the determination of vital cellular factors that affect prime editing in bacteria. Genetic screening of 129 Escherichia coli transposon mutants identified sbcB, a 3MODIFIER LETTER PRIME -> 5MODIFIER LETTER PRIME DNA exonuclease, as a key genetic determinant in impeding prime editing in E. coli, combinational deletions of which with two additional 3MODIFIER LETTER PRIME -> 5MODIFIER LETTER PRIME DNA exonucleases, xseA and exoX, drastically enhanced the prime editing efficiency by up to 100-fold. Efficient prime editing in wild-type E. coli can be achieved by simultaneously inhibiting the DNA exonucleases via CRISPRi. Our results pave the way for versatile applications of prime editing for bacterial genome engineering.","Prime editing in bacteria is currently inefficient. Here the authors report BacPE, a versatile prime editing platform in Escherichia coli that works by inhibiting 3 '-> 5 ' DNA exonucleases, highlighting the intrinsic genetic factors that are adverse to efficient prime editing."]
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收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China (National Science Foundation of China)["2023YFC3403400","2022YFC3400200"] ; National Key R&D Program of China["LG-QS-202206-05","22277078","22077083","22207074"] ; National Natural Science Foundation of China["23HC1400800","22ZR1480100","22YF1428100"] ; Shanghai Committee of Science and Technology, China[KF-202303]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001152430000025
出版者	NATURE PORTFOLIO
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/349914
专题	物质科学与技术学院生命科学与技术学院生命科学与技术学院_PI研究组_黄行许组生命科学与技术学院_PI研究组_朱焕乎组物质科学与技术学院_PI研究组_季泉江组物质科学与技术学院_硕士生生命科学与技术学院_博士生物质科学与技术学院_博士生
通讯作者	Ji, Quanjiang
作者单位	1.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China 2.ShanghaiTech Univ, State Key Lab Adv Med Mat & Devices, Shanghai 201210, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 4.Ningbo Univ, Sch Marine Sci, Ningbo 315832, Zhejiang, Peoples R China 5.Zhejiang Lab, Hangzhou 311121, Zhejiang, Peoples R China 6.ShanghaiTech Univ, Gene Editing Ctr, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 7.Shanghai Clin Res & Trial Ctr, Shanghai 201210, Peoples R China
第一作者单位	物质科学与技术学院; 上海科技大学
通讯作者单位	物质科学与技术学院; 上海科技大学; 生命科学与技术学院
第一作者的第一单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Zhang, Hongyuan,Ma, Jiacheng,Wu, Zhaowei,et al. BacPE: a versatile prime-editing platform in bacteria by inhibiting DNA exonucleases[J]. NATURE COMMUNICATIONS,2024,15(1).
APA	Zhang, Hongyuan.,Ma, Jiacheng.,Wu, Zhaowei.,Chen, Xiaoyang.,Qian, Yangyang.,...&Ji, Quanjiang.(2024).BacPE: a versatile prime-editing platform in bacteria by inhibiting DNA exonucleases.NATURE COMMUNICATIONS,15(1).
MLA	Zhang, Hongyuan,et al."BacPE: a versatile prime-editing platform in bacteria by inhibiting DNA exonucleases".NATURE COMMUNICATIONS 15.1(2024).