In situ structure of actin remodeling during glucose-stimulated insulin secretion using cryo-electron tomography

doi:doi.org/10.1038/s41467-024-45648-7

	In situ structure of actin remodeling during glucose-stimulated insulin secretion using cryo-electron tomography
	Li, Weimin1,2 ; Li, Angdi1,2 ; Yu, Bing1,2 ; Zhang, Xiaoxiao1 ; Liu, Xiaoyan1 ; White, Kate 3; Stevens, Raymond1,2 ; Baumeister, Wolfgang1,4 ; Sali, Andrej 5,6,7; Jasnin, Marion 8; Sun, Liping1
	2024-02-12
发表期刊	NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year])
ISSN	2041-1723
发表状态	已发表
DOI	doi.org/10.1038/s41467-024-45648-7
摘要	Actin mediates insulin secretion in pancreatic β-cells through remodeling. Hampered by limited resolution, previous studies have offered an ambiguous depiction as depolymerization and repolymerization. We report the in situ structure of actin remodeling in INS-1E β-cells during glucose-stimulated insulin secretion at nanoscale resolution. After remodeling, the actin filament network at the cell periphery exhibits three marked differences: 12% of actin filaments reorient quasi-orthogonally to the ventral membrane; the filament network mainly remains as cell-stabilizing bundles but partially reconfigures into a less compact arrangement; actin filaments anchored to the ventral membrane reorganize from a “netlike” to a “blooming” architecture. Furthermore, the density of actin filaments and microtubules around insulin secretory granules decreases, while actin filaments and microtubules become more densely packed. The actin filament network after remodeling potentially precedes the transport and release of insulin secretory granules. These findings advance our understanding of actin remodeling and its role in glucose-stimulated insulin secretion.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/349684
专题	生命科学与技术学院_博士生 iHuman研究所 iHuman研究所_特聘教授组_Andrej Sali组 iHuman研究所_特聘教授组_Raymond Stevens组 iHuman研究所_公共科研平台_动物细胞培养和蛋白纯化平台 iHuman研究所_特聘教授组_Wolfgang Baumeister组
通讯作者	Baumeister, Wolfgang; Sali, Andrej; Jasnin, Marion; Sun, Liping
作者单位	1.iHuman Institute, ShanghaiTech University, Shanghai, 201210, China 2.School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China 3.Department of Chemistry, Bridge Institute, USC Michelson Center for Convergent Bioscience, University of Southern California, Los Angeles, CA 90089, USA 4.Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany 5.Quantitative Biosciences Institute, University of California, San Francisco, San Francisco, CA 94158, USA 6.Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA 94158, USA 7.Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA 8.Helmholtz Pioneer Campus, Helmholtz Munich, 85764 Neuherberg, Germany; Department of Chemistry, Technical University of Munich, 85748 Garching, Germany
第一作者单位	iHuman研究所; 生命科学与技术学院
通讯作者单位	iHuman研究所
第一作者的第一单位	iHuman研究所
推荐引用方式 GB/T 7714	Li, Weimin,Li, Angdi,Yu, Bing,et al. In situ structure of actin remodeling during glucose-stimulated insulin secretion using cryo-electron tomography[J]. NATURE COMMUNICATIONS,2024.
APA	Li, Weimin.,Li, Angdi.,Yu, Bing.,Zhang, Xiaoxiao.,Liu, Xiaoyan.,...&Sun, Liping.(2024).In situ structure of actin remodeling during glucose-stimulated insulin secretion using cryo-electron tomography.NATURE COMMUNICATIONS.
MLA	Li, Weimin,et al."In situ structure of actin remodeling during glucose-stimulated insulin secretion using cryo-electron tomography".NATURE COMMUNICATIONS (2024).