No observable guide-RNA-independent off-target mutation induced by prime editor

doi:10.1101/2021.04.09.439109

	No observable guide-RNA-independent off-target mutation induced by prime editor
	Gao, R.1,2,3,4 ; Fu, Z.-c.5; Li, X.1,2,3,4 ; Wang, Y.5; Wei, J.; Li, G.1,2,3,4 ; Wang, L.1,2,3,4 ; Wu, J.1,2,3 ; Xue, W.5; Huang, X.1,2,3 ; Yang, L.5; Chen, J.1,2,3
	2021-04-09
状态	已发表
摘要	Prime editor (PE) has been recently developed to induce efficient and precise ontarget editing, whereas its guide RNA (gRNA)-independent off-target effects remain unknown. Here, we used whole-genome and whole-transcriptome sequencing to determine gRNA-independent off-target mutations in cells expanded from single colonies, in which PE generated precise editing at on-target sites. We found that PE triggered no observable gRNA-independent off-target mutation genome-wide or transcriptome-wide in transfected human cells, highlighting its high specificity.
语种	英语
DOI	10.1101/2021.04.09.439109
相关网址	查看原文
出处	bioRxiv
收录类别	PPRN.PPRN
WOS记录号	PPRN:8848175
WOS类目	Biochemistry & Molecular Biology
文献类型	预印本
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/348523
专题	生命科学与技术学院_博士生生命科学与技术学院生命科学与技术学院_PI研究组_黄鹏羽组生命科学与技术学院_PI研究组_陈佳组物质科学与技术学院_公共科研平台_分析测试平台生命科学与技术学院_硕士生
通讯作者	Huang, X.; Yang, L.; Chen, J.
作者单位	1.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 2.Shanghai Tech Univ, Gene Editing Ctr, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 3.Shanghai Inst Biochem & Cell Biol, Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai 200031, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Univ Chinese Acad Sci, MPG Partner Inst Computat Biol, Shanghai Inst Nutr & Hlth, Chinese Acad Sci, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Gao, R.,Fu, Z.-c.,Li, X.,et al. No observable guide-RNA-independent off-target mutation induced by prime editor. 2021.