CTP synthase does not form cytoophidia in<italic> Drosophila</italic> interfollicular stalks

doi:10.1101/2021.12.02.470862

	CTP synthase does not form cytoophidia in Drosophila interfollicular stalks
	Wu, Z.1 ; Liu, J.-l.1,2
	2021-12-03
状态	已发表
摘要	CTP synthase (CTPS) catalyzes the final step of de novo synthesis of the nucleotide CTP. In 2010, CTPS has been found to form filamentous structures termed cytoophidia in Drosophila follicle cells and germline cells. Subsequently, cytoophidia have been reported in many species across three domains of life: bacteria, eukaryotes and archaea. Forming cytoophidia appears to be a highly conserved and ancient property of CTPS. To our surprise, here we find that polar cells and stalk cells, two specialized types of cells composing Drosophila interfollicular stalks, do not possess obvious cytoophidia. Moreover, we show that Myc level is low in these two types of cells, supporting the idea that Myc regulates cytoophidium assembly. Treatment with a glutamine analog, 6-diazo-5-oxo-l-norleucine (DON), increases cytoophidium assembly in main follicle cells, but not in polar cells or stalk cells. Our findings provide an interesting paradigm for the in vivo study of cytoophidium assembly and disassembly among different populations of follicle cells.
关键词	CTP synthase cytoophidium Drosophila stalk cell polar cell
语种	英语
DOI	10.1101/2021.12.02.470862
相关网址	查看原文
出处	bioRxiv
收录类别	PPRN.PPRN
WOS记录号	PPRN:9504707
WOS类目	Developmental Biology
资助项目	ShanghaiTech University["MC_UU_12021/3","31771490"] ; UK Medical Research Council[MC_U137788471]
文献类型	预印本
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/348512
专题	生命科学与技术学院生命科学与技术学院_PI研究组_刘冀珑组生命科学与技术学院_本科生
通讯作者	Liu, J.-l.
作者单位	1.Shanghai Tech Univ, Sch Life Sci & Technol, 230 Haike Rd, Shanghai 201210, Peoples R China 2.Univ Oxford, Dept Physiol, Anat & Genet, South Parks Rd, Oxford OX1 3PT, Oxfordshire, England
推荐引用方式 GB/T 7714	Wu, Z.,Liu, J.-l.. CTP synthase does not form cytoophidia in Drosophila interfollicular stalks. 2021.