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	GTP as an enzymatic synchronizer
	Guo, C.-j.; Zhou, X.; Liu, J.-l.
	2022-09-24
状态	已发表
摘要	Synchronizer is the key component of modern automobile gearbox, which coordinates the deceleration and acceleration of two groups of gears. In industry, synchronizer is composed of several components with fine structure. Here, we show that guanosine triphosphate (GTP) has the property of enzyme synchronizer. Through cryo-electron microscopy (cryo-EM) and 3D variability analysis, we obtain the full spectrum conformation of CTP synthase at near atomic resolution. In the catalytic process of CTP synthase, we find that GTP synchronizes the reaction of two independent domains, namely, glutamine-dependent aminotransferase (GAT) domain and amidoligase (AL) domain. The wing structure acts as a striker, and then stimulates the intermediate ammonia to complete the catalytic cycle through the internal gas channel. Different from being degraded to regulate tubulins or G-proteins, GTP acts as an enzymatic synchronizer to coordinate the reactions catalyzed by the two independent domains of CTP synthase.
关键词	CTP synthase GTP glutamine-dependent amidotransferase amidoligase synchronizer
语种	英语
DOI	10.1101/2022.09.24.509316
相关网址	查看原文
出处	bioRxiv
收录类别	PPRN.PPRN
WOS记录号	PPRN:16896797
WOS类目	Biochemistry & Molecular Biology
资助项目	Ministry of Science and Technology of China["20JC1410500","31771490","2021YFA0804701"]
文献类型	预印本
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/348488
专题	生命科学与技术学院 生命科学与技术学院_PI研究组_刘冀珑组 生命科学与技术学院_博士生
通讯作者	Liu, J.-l.
作者单位	ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Guo, C.-j.,Zhou, X.,Liu, J.-l.. GTP as an enzymatic synchronizer. 2022.

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