Configurable Compartmentation Enables<italic> In vitro</italic> Reconstitution of Sustained Synthetic Biology Systems

doi:10.1101/2022.03.19.484972

	Configurable Compartmentation Enables In vitro Reconstitution of Sustained Synthetic Biology Systems
	Li, Luyao1 ; Zhang, R.1 ; Tian, Xintong1 ; Li, T.2; Pu, Bingchun 3; Ma, Conghui1 ; Ba, F.1 ; Xiong, Chenwei1 ; Shi, Yufeng 2; Li, J.1 ; Keasling, Jay 4,5,6,7; Zhang, Jingwei1,2 ; Liu, Yifan1
	2022-05-11
状态	已发表
摘要	The compartmentalized and communicative nature of biological cells contributes to the complexity and endurance of living organisms. Current in vitro compartmentalization systems such as droplet emulsions reproduce the compartmentalization property of cells yet fail to recapture the configurability of cellular communication with the environment. To mimic biological cells a step further and expand the capabilities of in vitro compartmentalization, we present here a general strategy that inherits the passive transport phenomenon of biology. The strategy incorporates layered, micrometer-sized, hydrogel-based compartments featuring configurability in composition, functionality, and selective permeability of biomolecules. We demonstrated the unique advantage of our strategy in two scenarios of synthetic biology. First, a compartmentalized cell-free protein synthesis system was reconstituted that could support multiple rounds of reactions. Second, we constructed living bacteria-based biosensors in the hydrogel compartments, which could achieve long-lasting functioning with markedly enhanced fitness in complex environments. Looking forward, our strategy should be widely applicable for constructing complex, robust, and sustained in vitro synthetic molecular and cellular systems, paving the way for their practical applications.
关键词	Synthetic Biology Cell-free Protein Synthesis Biosensor Biomaterial Compartmentalization
语种	英语
DOI	10.1101/2022.03.19.484972
相关网址	查看原文
出处	bioRxiv
收录类别	PPRN.PPRN
WOS记录号	PPRN:9265344
WOS类目	Biology
资助项目	National Key R&D Program of China["19PJ1401600","61904105","2020YFA0803800"] ; National Natural Science Foundation of China[32070948] ; Shanghai Pujiang Talent Program[91942313]
文献类型	预印本
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/348445
专题	物质科学与技术学院物质科学与技术学院_硕士生生命科学与技术学院_博士生物质科学与技术学院_博士生物质科学与技术学院_PI研究组_李军组物质科学与技术学院_PI研究组_刘一凡组
通讯作者	Li, J.; Zhang, Jingwei; Liu, Yifan
作者单位	1.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China 2.Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200438, Peoples R China 3.Shanghai Jiao Tong Univ Sch Med, Dept Immunol & Microbiol, Shanghai 200025, Peoples R China 4.Joint BioEnergy Inst, Emeryville, CA 94608, USA 5.Lawrence Berkeley Natl Lab, Biol Syst & Engn Div, Berkeley, CA 94720, USA 6.Univ Calif Berkeley, Dept Chem & Biomol Engn, Berkeley, CA 94720, USA 7.Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720, USA
推荐引用方式 GB/T 7714	Li, Luyao,Zhang, R.,Tian, Xintong,et al. Configurable Compartmentation Enables In vitro Reconstitution of Sustained Synthetic Biology Systems. 2022.