Engineering <italic>Escherichia coli </italic>to utilize erythritol as sole carbon source

doi:10.1101/2022.10.03.510612

	Engineering Escherichia coli to utilize erythritol as sole carbon source
	Ba, Fang; Ji, Xiangyang; Huang, Shuhui; Zhang, Yufei; Liu, Wan-Qiu; Liu, Yifan; Ling, Shengjie; Li, Jian
	2022-11-09
状态	已发表
摘要	Erythritol, one of the natural sugar alcohols, is widely used as a sugar substitute sweetener in food industries. Human themselves are not able to catabolize erythritol and their gut microbes lack related catabolic pathways either to metabolize erythritol. Here, we engineer Escherichia coli to utilize erythritol as sole carbon source aiming for defined applications. First, we isolate the erythritol metabolic gene cluster and experimentally characterize the erythritol-binding transcriptional repressor and its DNA-binding site. Transcriptome analysis suggests that carbohydrate metabolism-related genes in the engineered E. coli are overall upregulated, which then guides the selection of four genes for overexpression that notably enhances cell growth. Finally, engineered E. coli strains can be used as a living detector to distinguish erythritolcontaining soda soft drinks and can grow in the simulated intestinal fluid supplemented with erythritol. We expect our work will inspire the engineering of more hosts to respond and utilize erythritol for broad applications in metabolic engineering, synthetic biology, and biomedical engineering.
语种	英语
DOI	10.1101/2022.10.03.510612
相关网址	查看原文
出处	bioRxiv
收录类别	PPRN.PPRN
WOS记录号	PPRN:22834539
WOS类目	Biology
资助项目	National Natural Science Foundation of China["31971348","SYLDX0292022"] ; Double First-Class Initiative Fund of ShanghaiTech University[32171427]
文献类型	预印本
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/348420
专题	物质科学与技术学院物质科学与技术学院_PI研究组_凌盛杰组物质科学与技术学院_PI研究组_李健组物质科学与技术学院_博士生物质科学与技术学院_PI研究组_刘一凡组
通讯作者	Li, Jian
作者单位	Shanghai Tech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Ba, Fang,Ji, Xiangyang,Huang, Shuhui,et al. Engineering Escherichia coli to utilize erythritol as sole carbon source. 2022.