Cytoophidia influence cell cycle and size in <italic>Schizosaccharomyces pombe</italic>

doi:10.1101/2023.10.15.562340

	Cytoophidia influence cell cycle and size in Schizosaccharomyces pombe
	Deng, Ruolan 1,2,3; Li, Yi-Lan1,2,3 ; Liu, Ji-Long1,4
	2023-10-16
状态	已发表
摘要	CTP synthase (CTPS) forms cytoophidia in all three domains of life. Here we focus on the function of cytoophidia in cell proliferation using Schizosaccharomyces pombe as a model system. We find that converting His359 of CTPS into Ala359 leads to cytoophidium disassembly. By reducing the level of CTPS protein or specific point mutations, the loss of cytoophidia prolongs the G2 phase and expands cell size. In addition, the loss-filament mutant of CTPS leads to a decrease in the expression of genes related to G2/M transition and cell growth, including slm9. The overexpression of slm9 alleviates the G2 phase elongation and cell size enlargement induced by CTPS loss-filament mutant. Overall, our results connect cytoophidia with cell cycle and cell size control in Schizosaccharomyces pombe.
关键词	cell cycle cell size CTP synthase cytoophidium G2 phase Schizosaccharomyces pombe slm9
语种	英语
DOI	10.1101/2023.10.15.562340
相关网址	查看原文
出处	bioRxiv
收录类别	PPRN.PPRN
WOS记录号	PPRN:85608742
WOS类目	Cell Biology
资助项目	ShanghaiTech University["31771490","MC_UU_12021/3"] ; National Natural Science Foundation of China[MC_U137788471]
文献类型	预印本
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/348114
专题	生命科学与技术学院生命科学与技术学院_PI研究组_刘冀珑组生命科学与技术学院_博士生
通讯作者	Liu, Ji-Long
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, Shanghai 200031, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Univ Oxford, Dept Physiol Anat & Genet, Oxford OX1 3PT, England
推荐引用方式 GB/T 7714	Deng, Ruolan,Li, Yi-Lan,Liu, Ji-Long. Cytoophidia influence cell cycle and size in Schizosaccharomyces pombe. 2023.