Joint efforts of replicative helicase and SSB ensure inherent replicative tolerance of G-quadruplex

doi:10.1002/advs.202307696

	Joint efforts of replicative helicase and SSB ensure inherent replicative tolerance of G-quadruplex
	Guo, Lijuan1 ; Bao, Yanling1 ; Zhao, Yilin1 ; Ren, Zhiyun1 ; Bi, Lulu1 ; Zhang, Xia1 ; Liu, Cong 2; Hou, Xi-Miao 3; Wang, Michelle D 4; Sun, Bo1
	2023-12-21
发表期刊	ADVANCED SCIENCE (IF:14.3[JCR-2023],16.3[5-Year])
ISSN	2198-3844
EISSN	2198-3844
卷号	11 期号:9 页码:2307696
发表状态	已发表
DOI	10.1002/advs.202307696
摘要	G-quadruplex (G4) is a four-stranded noncanonical DNA structure that has long been recognized as a potential hindrance to DNA replication. However, how replisomes effectively deal with G4s to avoid replication failure is still obscure. Here, using single-molecule and ensemble approaches, the consequence of the collision between bacteriophage T7 replisome and an intramolecular G4 located on either the leading or lagging strand is examined. It is found that the adjacent fork junctions induced by G4 formation incur the binding of T7 DNA polymerase (DNAP). In addition to G4, these inactive DNAPs present insuperable obstacles, impeding the progression of DNA synthesis. Nevertheless, T7 helicase can dismantle them and resolve lagging-strand G4s, paving the way for the advancement of the replication fork. Moreover, with the assistance of the single-stranded DNA binding protein (SSB) gp2.5, T7 helicase is also capable of maintaining a leading-strand G4 structure in an unfolded state, allowing for a fraction of T7 DNAPs to synthesize through without collapse. These findings broaden the functional repertoire of a replicative helicase and underscore the inherent G4 tolerance of a replisome. © 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.
关键词	DNA replications DNA structure Ensemble approaches G-quadruplexes Helicases Lagging-strand Polymerase Replisomes Single molecule SSB
收录类别	SCI ; EI
语种	英语
出版者	John Wiley and Sons Inc
EI入藏号	20235215267097
EI主题词	DNA
EI分类号	461.2 Biological Materials and Tissue Engineering
原始文献类型	Article in Press
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/347877
专题	生命科学与技术学院生命科学与技术学院_PI研究组_孙博组生命科学与技术学院_博士生生命科学与技术学院_本科生
共同第一作者	Bao, Yanling
通讯作者	Sun, Bo
作者单位	1.School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China 2.Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 201210, China 3.College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China 4.Department of Physics, Laboratory of Atomic and Solid State Physics, Cornell University, Ithaca, New York 14853, USA
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Guo, Lijuan,Bao, Yanling,Zhao, Yilin,et al. Joint efforts of replicative helicase and SSB ensure inherent replicative tolerance of G-quadruplex[J]. ADVANCED SCIENCE,2023,11(9):2307696.
APA	Guo, Lijuan.,Bao, Yanling.,Zhao, Yilin.,Ren, Zhiyun.,Bi, Lulu.,...&Sun, Bo.(2023).Joint efforts of replicative helicase and SSB ensure inherent replicative tolerance of G-quadruplex.ADVANCED SCIENCE,11(9),2307696.
MLA	Guo, Lijuan,et al."Joint efforts of replicative helicase and SSB ensure inherent replicative tolerance of G-quadruplex".ADVANCED SCIENCE 11.9(2023):2307696.