DROPLET-BASED ULTRA-HIGH-THROUGHPUT MICROBIAL SINGLE-CELL WHOLE-GENOME AMPLIFICATION AND BARCODING

	DROPLET-BASED ULTRA-HIGH-THROUGHPUT MICROBIAL SINGLE-CELL WHOLE-GENOME AMPLIFICATION AND BARCODING
	Li, Jie; Zhang, Rong; Liu, Yifan
	2022
会议录名称	MICROTAS 2022 - 26TH INTERNATIONAL CONFERENCE ON MINIATURIZED SYSTEMS FOR CHEMISTRY AND LIFE SCIENCES
页码	448-449
摘要	In recent years, increasing research efforts have provided us with tremendous insights into microbial communities. However, limited by current technologies, our understanding of the diverse communities of microbes is inadequate. Here we report a method of amplifying and barcoding the whole genome of thousands of individual microbes. The method takes advantage of droplet microfluidics to integrate encapsulation of single microbial cells, whole genome amplification, and indexing of each amplified genome at ultra-high throughput. Our approach may offer new insights into microbial species identification, and pave the way of revealing microbial dark matter. © 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
会议录编者/会议主办者	Chemical and Biological Microsystems Society (CBMS)
关键词	Bacteria Genes Microfluidics Current technology Diverse community Droplet microfluidics High-throughput Microbial communities Microbiome Research efforts Single cells Ultra-high Whole genome amplifications
会议名称	26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022
会议地点	Hybrid, Hangzhou, China
会议日期	October 23, 2022 - October 27, 2022
收录类别	EI
语种	英语
出版者	Chemical and Biological Microsystems Society
EI入藏号	20234515027485
EI主题词	Drops
EI分类号	461.2 Biological Materials and Tissue Engineering ; 632.5.1 Microfluidics
原始文献类型	Conference article (CA)
文献类型	会议论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/346430
专题	物质科学与技术学院物质科学与技术学院_博士生物质科学与技术学院_PI研究组_刘一凡组
作者单位	Division of Chemistry and Physical Biology, School of Physical Science and Technology, ShanghaiTech University, Shanghai; 201210, China
第一作者单位	物质科学与技术学院
第一作者的第一单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Li, Jie,Zhang, Rong,Liu, Yifan. DROPLET-BASED ULTRA-HIGH-THROUGHPUT MICROBIAL SINGLE-CELL WHOLE-GENOME AMPLIFICATION AND BARCODING[C]//Chemical and Biological Microsystems Society (CBMS):Chemical and Biological Microsystems Society,2022:448-449.