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ShanghaiTech University Knowledge Management System
| BARCODED COMBINATORIAL SCREENING FOR HIGHLY EFFICIENT OPTIMIZATION OF CELL-FREE PROTEIN SYNTHESIS SYSTEMS IN DROPLETS | |
| 2022 | |
| 会议录名称 | MICROTAS 2022 - 26TH INTERNATIONAL CONFERENCE ON MINIATURIZED SYSTEMS FOR CHEMISTRY AND LIFE SCIENCES |
| 页码 | 134-135 |
| 摘要 | We present a novel droplet-based barcoded combinatorial Screening (BaCon) platform which enables rapid, massively parallel, and ultra-high-throughput screening of optimal conditions of cell-free protein expression systems. With this platform, we first demonstrate the ability of BaCon in recognizing microdroplets under various reaction conditions using fluorescent labeling. We further apply this technique to optimize a cell-free protein synthesis system. Broadly, BaCon can recognize multiparameter mixtures possessing any optically assayable function or morphological change, including optimization of reagent and reaction conditions, combinatorial drug screening, directed evolution, and biochemical assays. © 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved. |
| 会议录编者/会议主办者 | Chemical and Biological Microsystems Society (CBMS) |
| 关键词 | Bar codes Biosynthesis Cells Cytology Fluorescence Proteins Cell free protein synthesis Combinatorial screenings Droplet microfluidics Efficient optimisation Fluorescent barcode High throughput screening Massively parallels Optimal conditions Reaction conditions Ultra-high |
| 会议名称 | 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 |
| 会议地点 | Hybrid, Hangzhou, China |
| 会议日期 | October 23, 2022 - October 27, 2022 |
| 收录类别 | EI |
| 语种 | 英语 |
| 出版者 | Chemical and Biological Microsystems Society |
| EI入藏号 | 20234515027583 |
| EI主题词 | Drops |
| EI分类号 | 461.2 Biological Materials and Tissue Engineering ; 461.8 Biotechnology ; 461.9 Biology ; 723.2 Data Processing and Image Processing ; 741.1 Light/Optics ; 801.2 Biochemistry ; 802.2 Chemical Reactions ; 804.1 Organic Compounds |
| 原始文献类型 | Conference article (CA) |
| 文献类型 | 会议论文 |
| 条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/346429 |
| 专题 | 物质科学与技术学院 物质科学与技术学院_PI研究组_李健组 物质科学与技术学院_硕士生 物质科学与技术学院_博士生 |
| 作者单位 | School of Physical Science and Technology, ShanghaiTech University, Shanghai; 201210, China |
| 第一作者单位 | 物质科学与技术学院 |
| 第一作者的第一单位 | 物质科学与技术学院 |
| 推荐引用方式 GB/T 7714 | Zhu, Jiawei,Meng, Yaru,Ma, Conghui,et al. BARCODED COMBINATORIAL SCREENING FOR HIGHLY EFFICIENT OPTIMIZATION OF CELL-FREE PROTEIN SYNTHESIS SYSTEMS IN DROPLETS[C]//Chemical and Biological Microsystems Society (CBMS):Chemical and Biological Microsystems Society,2022:134-135. |
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