ELECTRICAL GENERATION OF ION CONCENTRATION GRADIENT IN HYDROGEL MICROFLUIDIC DEVICE

	ELECTRICAL GENERATION OF ION CONCENTRATION GRADIENT IN HYDROGEL MICROFLUIDIC DEVICE
	Xiong, Chenwei; Li, Jie; Zhu, Yuewei; Chen, Long; Zhang, Rong; Liu, Yifan
	2022
会议录名称	MICROTAS 2022 - 26TH INTERNATIONAL CONFERENCE ON MINIATURIZED SYSTEMS FOR CHEMISTRY AND LIFE SCIENCES
页码	813-814
摘要	In nature, many cellular phenomena are driven by the dynamic chemical gradient in the surrounding microenvironment. In this context, establishing a biological-relevant and adjustable chemical gradient in vitro is essential for the study of cellular functions. However, it is challenging to alter the chemical gradient rapidly and dynamically using dilution-based methods. In this paper, we develop a facile approach to realizing dynamically adjustable chemical gradients in a microfluidic device. The approach takes advantage of the ion concentration polarization effect occurring around a perm-selective hydrogel situated in a microchannel. By simply adjusting the applied voltage, the gradient of ions and charged molecules can be controlled from -0.0082 to 0.0081 μM/μm, a range relative to biological conditions. We are currently using this simple and generalizable method of establishing dynamic chemical gradients to study the chemotaxis behavior of bacteria. © 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
会议录编者/会议主办者	Chemical and Biological Microsystems Society (CBMS)
关键词	Biochemistry Concentration (process) Fluidic devices Hydrogels Ions Polarization Cellular function Cellulars Chemical gradients Concentration- gradients Electrical generation In-vitro Ion concentration polarizations Ion concentrations Microenvironments Microfluidics devices
会议名称	26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022
会议地点	Hybrid, Hangzhou, China
会议日期	October 23, 2022 - October 27, 2022
收录类别	EI
语种	英语
出版者	Chemical and Biological Microsystems Society
EI入藏号	20234515027566
EI主题词	Microfluidics
EI分类号	632.2 Hydraulic Equipment and Machinery ; 632.5.1 Microfluidics ; 732.1 Control Equipment ; 801.2 Biochemistry ; 801.3 Colloid Chemistry ; 804 Chemical Products Generally
原始文献类型	Conference article (CA)
文献类型	会议论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/346428
专题	物质科学与技术学院物质科学与技术学院_硕士生物质科学与技术学院_博士生物质科学与技术学院_PI研究组_刘一凡组物质科学与技术学院_PI研究组_翟晓芳组
作者单位	School of Physical Science and Technology, ShanghaiTech University, Shanghai; 201210, China
第一作者单位	物质科学与技术学院
第一作者的第一单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Xiong, Chenwei,Li, Jie,Zhu, Yuewei,et al. ELECTRICAL GENERATION OF ION CONCENTRATION GRADIENT IN HYDROGEL MICROFLUIDIC DEVICE[C]//Chemical and Biological Microsystems Society (CBMS):Chemical and Biological Microsystems Society,2022:813-814.