ShanghaiTech University Knowledge Management System
| Logarithmic-dilution-based droplet digital PCR for high-dynamic-range quantification of clinical-relevant viral pathogens | |
| 2022 | |
| 会议录名称 | MICROTAS 2022 - 26TH INTERNATIONAL CONFERENCE ON MINIATURIZED SYSTEMS FOR CHEMISTRY AND LIFE SCIENCES |
| 页码 | 602-603 |
| 发表状态 | 已发表 |
| 摘要 | We report logarithmic-dilution-based droplet digital PCR (LD3 PCR), a customized yet generalizable droplet digital PCR (ddPCR) approach that features a significantly extended dynamic range over 7 orders of magnitude. Such a performance is 3 orders higher than conventional ddPCR and close to well-established quantitative PCR (qPCR). LD3 PCR was used for HPV16 genotyping in clinical-relevant samples. The results show that LD3 PCR maintains accuracy and robustness in detecting 40 to 1.98×107 copies/μl templates. We anticipate that LD3 PCR may be broadly applied in quantifying clinical-relevant viral pathogens. © 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved. |
| 会议录编者/会议主办者 | Chemical and Biological Microsystems Society (CBMS) |
| 关键词 | Digital microfluidics Pathogens Polymerase chain reaction Digital PCR Dynamic range Extended dynamic ranges High dynamic range In vitro diagnose In-vitro Orders of magnitude PCR approach Performance Quantitative PCR |
| 会议名称 | 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 |
| 会议地点 | Hybrid, Hangzhou, China |
| 会议日期 | October 23, 2022 - October 27, 2022 |
| 收录类别 | EI |
| 语种 | 英语 |
| 出版者 | Chemical and Biological Microsystems Society |
| EI入藏号 | 20234515027362 |
| EI主题词 | Drops |
| EI分类号 | 461.9 Biology ; 632.5.1 Microfluidics ; 801.2 Biochemistry |
| 原始文献类型 | Conference article (CA) |
| 文献类型 | 会议论文 |
| 条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/346427 |
| 专题 | 物质科学与技术学院 物质科学与技术学院_硕士生 物质科学与技术学院_博士生 物质科学与技术学院_PI研究组_刘一凡组 |
| 作者单位 | School of Physical Science and Technology, ShanghaiTech University, Shanghai; 201210, China |
| 第一作者单位 | 物质科学与技术学院 |
| 第一作者的第一单位 | 物质科学与技术学院 |
| 推荐引用方式 GB/T 7714 | Shi, Qingyuan,Li, Jie,Zhang, Rong,et al. Logarithmic-dilution-based droplet digital PCR for high-dynamic-range quantification of clinical-relevant viral pathogens[C]//Chemical and Biological Microsystems Society (CBMS):Chemical and Biological Microsystems Society,2022:602-603. |
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