Time-resolved proteomic profiling reveals compositional and functional transitions across the stress granule life cycle

doi:10.1038/s41467-023-43470-1

	Time-resolved proteomic profiling reveals compositional and functional transitions across the stress granule life cycle
	Shuyao Hu; Yufeng Zhang; Qianqian Yi; Cuiwei Yang; Yanfen Liu; Yun Bai
	2023
发表期刊	NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year])
ISSN	2041-1723
EISSN	2041-1723
卷号	14 期号:1 页码:7782
发表状态	已发表
DOI	10.1038/s41467-023-43470-1
摘要	Stress granules (SGs) are dynamic, membrane-less organelles. With their formation and disassembly processes characterized, it remains elusive how compositional transitions are coordinated during prolonged stress to meet changing functional needs. Here, using time-resolved proteomic profiling of the acute to prolonged heat-shock SG life cycle, we identify dynamic SG proteins, further segregated into early and late proteins. Comparison of different groups of SG proteins suggests that their biochemical properties help coordinate SG compositional and functional transitions. In particular, early proteins, with high phase-separation-propensity, drive the rapid formation of the initial SG platform, while late proteins are subsequently recruited as discrete modules to further functionalize SGs. This model, supported by immunoblotting and immunofluorescence imaging, provides a conceptual framework for the compositional transitions throughout the acute to prolonged SG life cycle. Additionally, an early SG constituent, non-muscle myosin II, is shown to promote SG formation by increasing SG fusion, underscoring the strength of this dataset in revealing the complexity of SG regulation.
URL	查看原文
收录类别	SCI
语种	英语
资助项目	National Science Foundation of China["31670756","32070697"] ; Ministry of Science and Technology of the People's Republic of China[2016YFA0500901]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001316824700021
出版者	NATURE PORTFOLIO
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/345918
专题	生命科学与技术学院_博士生生命科学与技术学院_PI研究组_白云组生命科学与技术学院_PI研究组_刘艳芬组
通讯作者	Shuyao Hu; Yanfen Liu; Yun Bai
作者单位	ShanghaiTech University
推荐引用方式 GB/T 7714	Shuyao Hu,Yufeng Zhang,Qianqian Yi,et al. Time-resolved proteomic profiling reveals compositional and functional transitions across the stress granule life cycle[J]. NATURE COMMUNICATIONS,2023,14(1):7782.
APA	Shuyao Hu,Yufeng Zhang,Qianqian Yi,Cuiwei Yang,Yanfen Liu,&Yun Bai.(2023).Time-resolved proteomic profiling reveals compositional and functional transitions across the stress granule life cycle.NATURE COMMUNICATIONS,14(1),7782.
MLA	Shuyao Hu,et al."Time-resolved proteomic profiling reveals compositional and functional transitions across the stress granule life cycle".NATURE COMMUNICATIONS 14.1(2023):7782.