mTOR-S6K1 pathway mediates cytoophidium assembly
Sun, Zhe1; Liu, Ji-Long1,2
2019-02
Source PublicationJOURNAL OF GENETICS AND GENOMICS
ISSN1673-8527
Volume46Issue:2Pages:65-74
Status已发表
DOI10.1016/j.jgg.2018.11.006
AbstractCTP synthase (CTPS), the rate-limiting enzyme in de novo CTP biosynthesis, has been demonstrated to assemble into evolutionarily conserved filamentous structures, termed cytoophidia, in Drosophila, bacteria, yeast and mammalian cells. However, the regulation and function of the cytoophidium remain elusive. Here, we provide evidence that the mechanistic target of rapamycin (mTOR) pathway controls cytoophidium assembly in mammalian and Drosophila cells. In mammalian cells, we find that inhibition of mTOR pathway attenuates cytoophidium formation. Moreover, CTPS cytoophidium assembly appears to be dependent on the mTOR complex 1 (mTORC1) mainly. In addition, knockdown of the mTORC1 downstream target S6K1 can inhibit cytoophidium formation, while overexpression of the constitutively active S6K1 reverses mTOR knockdown-induced cytoophidium disassembly. Finally, reducing mTOR protein expression results in a decrease of the length of cytoophidium in Drosophila follicle cells. Therefore, our study connects CTPS cytoophidium formation with the mTOR signaling pathway. (C) 2019, The Authors. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press.
KeywordmTOR Cytoophidium CTP synthase Colorectal cancer cell Drosophila
Indexed BySCI ; CSCD
Language英语
Funding ProjectUK Medical Research Council[MC_UU_12021/3] ; UK Medical Research Council[MC_U137788471]
WOS Research AreaBiochemistry & Molecular Biology ; Genetics & Heredity
WOS SubjectBiochemistry & Molecular Biology ; Genetics & Heredity
WOS IDWOS:000464135500001
PublisherSCIENCE PRESS
WOS KeywordCYTIDINE TRIPHOSPHATE SYNTHETASE ; CTP SYNTHASE FORMS ; PROTEIN-KINASE-A ; PHASE-II ; SACCHAROMYCES-CEREVISIAE ; PHOSPHATIDYLCHOLINE SYNTHESIS ; NUCLEOTIDE SYNTHESIS ; PHOSPHORYLATION ; MTOR ; DROSOPHILA
Original Document TypeArticle
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://kms.shanghaitech.edu.cn/handle/2MSLDSTB/34176
Collection生命科学与技术学院
生命科学与技术学院_PI研究组_刘冀珑组
Corresponding AuthorLiu, Ji-Long
Affiliation1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
2.Univ Oxford, Dept Physiol Anat & Genet, MRC Funct Genom Unit, Oxford OX1 3PT, England
First Author AffilicationSchool of Life Science and Technology
Corresponding Author AffilicationSchool of Life Science and Technology
First Signature AffilicationSchool of Life Science and Technology
Recommended Citation
GB/T 7714
Sun, Zhe,Liu, Ji-Long. mTOR-S6K1 pathway mediates cytoophidium assembly[J]. JOURNAL OF GENETICS AND GENOMICS,2019,46(2):65-74.
APA Sun, Zhe,&Liu, Ji-Long.(2019).mTOR-S6K1 pathway mediates cytoophidium assembly.JOURNAL OF GENETICS AND GENOMICS,46(2),65-74.
MLA Sun, Zhe,et al."mTOR-S6K1 pathway mediates cytoophidium assembly".JOURNAL OF GENETICS AND GENOMICS 46.2(2019):65-74.
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