ShanghaiTech University Knowledge Management System
| Progress and Prospects of Gene Editing in Pluripotent Stem Cells | |
| 2023-08-01 | |
| Source Publication | BIOMEDICINES
 |
| EISSN | 2227-9059 |
| Volume | 11Issue:8 |
| Status | 已发表 |
| DOI | 10.3390/biomedicines11082168 |
| Abstract | Applying programmable nucleases in gene editing has greatly shaped current research in basic biology and clinical translation. Gene editing in human pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), is highly relevant to clinical cell therapy and thus should be examined with particular caution. First, since all mutations in PSCs will be carried to all their progenies, off-target edits of editors will be amplified. Second, due to the hypersensitivity of PSCs to DNA damage, double-strand breaks (DSBs) made by gene editing could lead to low editing efficiency and the enrichment of cell populations with defective genomic safeguards. In this regard, DSB-independent gene editing tools, such as base editors and prime editors, are favored due to their nature to avoid these consequences. With more understanding of the microbial world, new systems, such as Cas-related nucleases, transposons, and recombinases, are also expanding the toolbox for gene editing. In this review, we discuss current applications of programmable nucleases in PSCs for gene editing, the efforts researchers have made to optimize these systems, as well as new tools that can be potentially employed for differentiation modeling and therapeutic applications. |
| Keyword | pluripotent stem cell induced pluripotent stem cell CRISPR-Cas9 base editor prime editor gene editing |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology ; Research & Experimental Medicine ; Pharmacology & Pharmacy |
| WOS Subject | Biochemistry & Molecular Biology ; Medicine, Research & Experimental ; Pharmacology & Pharmacy |
| WOS ID | WOS:001056653500001 |
| Publisher | MDPI |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/327926 |
| Collection | 生命科学与技术学院 生命科学与技术学院_PI研究组_林照博组 生命科学与技术学院_硕士生 |
| Corresponding Author | Lin, Chao-Po |
| Affiliation | ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China |
| First Author Affilication | School of Life Science and Technology |
| Corresponding Author Affilication | School of Life Science and Technology |
| First Signature Affilication | School of Life Science and Technology |
| Recommended Citation GB/T 7714 | Zhang, Zhenwu,Bao, Xinyu,Lin, Chao-Po. Progress and Prospects of Gene Editing in Pluripotent Stem Cells[J]. BIOMEDICINES,2023,11(8). |
| APA | Zhang, Zhenwu,Bao, Xinyu,&Lin, Chao-Po.(2023).Progress and Prospects of Gene Editing in Pluripotent Stem Cells.BIOMEDICINES,11(8). |
| MLA | Zhang, Zhenwu,et al."Progress and Prospects of Gene Editing in Pluripotent Stem Cells".BIOMEDICINES 11.8(2023). |
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