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ShanghaiTech University Knowledge Management System
| CATI: an efficient gene integration method for rodent and primate embryos by MMEJ suppression | |
| 2023-06-23 | |
| 发表期刊 | GENOME BIOLOGY (IF:10.1[JCR-2023],16.5[5-Year]) |
| ISSN | 1474-760X |
| 卷号 | 24期号:1 |
| 发表状态 | 已发表 |
| DOI | 10.1186/s13059-023-02987-w |
| 摘要 | The efficiency of homology-directed repair (HDR) plays a crucial role in the development of animal models and gene therapy. We demonstrate that microhomology-mediated end-joining (MMEJ) constitutes a substantial proportion of DNA repair during CRISPR-mediated gene editing. Using CasRx to downregulate a key MMEJ factor, Polymerase Q (Polq), we improve the targeted integration efficiency of linearized DNA fragments and single-strand oligonucleotides (ssODN) in mouse embryos and offspring. CasRX-assisted targeted integration (CATI) also leads to substantial improvements in HDR efficiency during the CRISPR/Cas9 editing of monkey embryos. We present a promising tool for generating monkey models and developing gene therapies for clinical trials. |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Shanghai Municipal Science and Technology Major Project[2018SHZDZX05] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB32060000] ; Basic Frontier Scientific Research Program of CAS[ZDBS-LY-SM019] ; National Natural Science Foundation of China Grant[ |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS类目 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS记录号 | WOS:001018633900001 |
| 出版者 | BMC |
| 引用统计 | 正在获取...
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| 文献类型 | 期刊论文 |
| 条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/317197 |
| 专题 | 生命科学与技术学院 生命科学与技术学院_硕士生 生命科学与技术学院_PI研究组_罗振革组 |
| 通讯作者 | Lu, Zongyang; Sun, Qiang; Liu, Zhen |
| 作者单位 | 1.Chinese Acad Sci, Inst Neurosci, Ctr Excellence Brain Sci & Intelligence Technol, State Key Lab Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China 2.Univ Chinese Acad Sci, 19A Yu Quan Rd, Beijing 100049, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 4.Shanghai Ctr Brain Sci & Brain Inspired Intelligen, Shanghai 201210, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Hongyu,Liu, Xingchen,Li, Lanxin,et al. CATI: an efficient gene integration method for rodent and primate embryos by MMEJ suppression[J]. GENOME BIOLOGY,2023,24(1). |
| APA | Chen, Hongyu.,Liu, Xingchen.,Li, Lanxin.,Tan, Qingtong.,Li, Shiyan.,...&Liu, Zhen.(2023).CATI: an efficient gene integration method for rodent and primate embryos by MMEJ suppression.GENOME BIOLOGY,24(1). |
| MLA | Chen, Hongyu,et al."CATI: an efficient gene integration method for rodent and primate embryos by MMEJ suppression".GENOME BIOLOGY 24.1(2023). |
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