Directing curli polymerization with DNA origami nucleators

doi:10.1038/s41467-019-09369-6

	Directing curli polymerization with DNA origami nucleators
	Mao, Xiuhai1,2,3,8 ; Li, Ke1 ; Liu, Mengmeng 4; Wang, Xinyu1 ; Zhao, Tianxin1 ; An, Bolin1 ; Cui, Mengkui1 ; Li, Yingfeng1 ; Pu, Jiahua1 ; Li, Jiang 2,3; Wang, Lihua 2,3,4; Lu, Timothy K.5; Fan, Chunhai 6,7; Zhong, Chao1
	2019-03-27
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
卷号	10
发表状态	已发表
DOI	10.1038/s41467-019-09369-6
摘要	The physiological or pathological formation of fibrils often relies on molecular-scale nucleators that finely control the kinetics and structural features. However, mechanistic understanding of how protein nucleators mediate fibril formation in cells remains elusive. Here, we develop a CsgB-decorated DNA origami (CB-origami) to mimic protein nucleators in Escherichia coli biofilm that direct curli polymerization. We show that CB-origami directs curli subunit CsgA monomers to form oligomers and then accelerates fibril formation by increasing the proliferation rate of primary pathways. Fibrils grow either out from (departure mode) or towards the nucleators (arrival mode), implying two distinct roles of CsgB: as nucleation sites and as trap sites to capture growing nanofibrils in vicinity. Curli polymerization follows typical stop-and-go dynamics but exhibits a higher instantaneous elongation rate compared with independent fibril growth. This origami nucleator thus provides an in vitro platform for mechanistically probing molecular nucleation and controlling directional fibril polymerization for bionanotechnology.
收录类别	SCI ; SCIE
语种	英语
资助项目	NSFC[U1532119] ; NSFC[21675167] ; NSFC[21834007] ; NSFC[21804088]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000462458900010
出版者	NATURE PUBLISHING GROUP
WOS关键词	AMYLOID FIBRILS ; PROTEIN ; CSGB ; KINETICS ; GROWTH
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/30633
专题	物质科学与技术学院_PI研究组_钟超组物质科学与技术学院_硕士生物质科学与技术学院_博士生
通讯作者	Fan, Chunhai; Zhong, Chao
作者单位	1.ShanghaiTech Univ, Sch Phys Sci & Technol, Mat & Phys Biol Div, Shanghai 201210, Peoples R China 2.Chinese Acad Sci, Div Phys Biol, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China 3.Chinese Acad Sci, Bioimaging Ctr, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China 4.East China Normal Univ, Sch Chem & Mol Engn, Shanghai Key Lab Green Chem & Chem Proc, 500 Dongchuan Rd, Shanghai 200241, Peoples R China 5.MIT, Synthet Biol Grp, Res Lab Elect, 77 Massachusetts Ave, Cambridge, MA 02139 USA 6.Shanghai Jiao Tong Univ, Renji Hosp, Sch Chem & Chem Engn, Sch Med, Shanghai 200240, Peoples R China 7.Shanghai Jiao Tong Univ, Renji Hosp, Inst Mol Med, Sch Med, Shanghai 200240, Peoples R China 8.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Inst Mol Med, Shanghai 200127, Peoples R China
第一作者单位	物质科学与技术学院
通讯作者单位	物质科学与技术学院
第一作者的第一单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Mao, Xiuhai,Li, Ke,Liu, Mengmeng,et al. Directing curli polymerization with DNA origami nucleators[J]. NATURE COMMUNICATIONS,2019,10.
APA	Mao, Xiuhai.,Li, Ke.,Liu, Mengmeng.,Wang, Xinyu.,Zhao, Tianxin.,...&Zhong, Chao.(2019).Directing curli polymerization with DNA origami nucleators.NATURE COMMUNICATIONS,10.
MLA	Mao, Xiuhai,et al."Directing curli polymerization with DNA origami nucleators".NATURE COMMUNICATIONS 10(2019).