Preparation of a monolithic column with a mixed-mode stationary phase of reversed-phase/hydrophilic interaction for capillary liquid chromatography

doi:10.1002/jssc.201800826

	Preparation of a monolithic column with a mixed-mode stationary phase of reversed-phase/hydrophilic interaction for capillary liquid chromatography
	Zhang, Meng1,2 ; Gong, Li 1; Liu, Guizhen1,2 ; Kang, Jingwu1,2
	2019-02
发表期刊	JOURNAL OF SEPARATION SCIENCE (IF:2.8[JCR-2023],2.7[5-Year])
ISSN	1615-9306
卷号	42 期号:3 页码:662-669
发表状态	已发表
DOI	10.1002/jssc.201800826
摘要	A monolithic capillary column with a mixed-mode stationary phase of reversedphase/ hydrophilic interaction chromatography was prepared for capillary liquid chromatography. Themonolith was created by an in-situ copolymerization of a homemade monomer N, N-dimethyl-N-acryloxyundecyl-N-(3-sulfopropyl) ammonium betaine and a crosslinker pentaerythritol triacrylate in a binary porogen agent consisting of methanol and isopropanol. The functional monomer was designed to have a highly polar zwitterionic sulfobetaine terminal group and a hydrophobic long alkyl chain moiety. The composition of the polymerization solution was systematically optimized to permit the best column performance. The columns were evaluated by using acidic, basic, polar neutral analytes, as well as a set of alkylbenzenes and Triton X100. Very good separations were obtained on the column with the mixed-mode stationary phase. It was demonstrated that the mixed-mode stationary phase displayed typic dual retention mechanisms of reversed-phase/ hydrophilic interaction liquid chromatography depending on the content of acetonitrile in the mobile phase. The method for column preparation is reproducible.
关键词	capillary liquid chromatography hydrophilic interaction mixed-mode stationary phases monolithic columns
收录类别	SCI ; SCIE ; EI
语种	英语
资助项目	Chinese Academy of Sciences (the Strategic Priority Research Program)[XDB20020200]
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000457651000003
出版者	WILEY-V C H VERLAG GMBH
WOS关键词	HYDROPHILIC-INTERACTION CHROMATOGRAPHY ; SEPARATION ; ACIDS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/30244
专题	物质科学与技术学院_博士生物质科学与技术学院_特聘教授组_康经武组物质科学与技术学院_硕士生
通讯作者	Kang, Jingwu
作者单位	1.Univ Chinese Acad Sci, Shanghai Inst Organ Chem, Ctr Excellence Mol Synth, State Key Lab Bioorgan & Nat Prod Chem, Shanghai, Peoples R China 2.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai, Peoples R China
第一作者单位	物质科学与技术学院
通讯作者单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Zhang, Meng,Gong, Li,Liu, Guizhen,et al. Preparation of a monolithic column with a mixed-mode stationary phase of reversed-phase/hydrophilic interaction for capillary liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2019,42(3):662-669.
APA	Zhang, Meng,Gong, Li,Liu, Guizhen,&Kang, Jingwu.(2019).Preparation of a monolithic column with a mixed-mode stationary phase of reversed-phase/hydrophilic interaction for capillary liquid chromatography.JOURNAL OF SEPARATION SCIENCE,42(3),662-669.
MLA	Zhang, Meng,et al."Preparation of a monolithic column with a mixed-mode stationary phase of reversed-phase/hydrophilic interaction for capillary liquid chromatography".JOURNAL OF SEPARATION SCIENCE 42.3(2019):662-669.