MiR-4465 directly targets PTEN to inhibit AKT/mTOR pathway-mediated autophagy

doi:10.1007/s12192-018-0946-6

	MiR-4465 directly targets PTEN to inhibit AKT/mTOR pathway-mediated autophagy
	Tao, Zhouteng 1,2; Feng, Chenxi 1; Mao, Chenmei 1; Ren, Jin 2; Tai, Yusi 2; Guo, Huijie2,3 ; Pu, Mei2,3 ; Zhou, Yang2,3 ; Wang, Guanghui 4,5; Wang, Mei 1
	2019-01
发表期刊	CELL STRESS & CHAPERONES (IF:3.3[JCR-2023],3.5[5-Year])
ISSN	1355-8145
卷号	24 期号:1 页码:105-113
发表状态	已发表
DOI	10.1007/s12192-018-0946-6
摘要	Autophagy plays an important role in maintaining cell function. Abnormal autophagy leads to cell dysfunction and is associated with many diseases such as tumors, immunodeficiency diseases, lysosomal storage disorders, and neurodegenerative diseases. Autophagy is precisely regulated, and PTEN plays an important role in regulating autophagy. As noncoding small RNAs, miRNAs play an important role in the fine regulation of cellular processes. However, the mechanism of the miRNA regulation of PTEN-related autophagy has not been fully elucidated. In this study, our results showed that miR-4465 significantly inhibited the expression of PTEN, upregulated phosphorylated AKT, and thereby inhibited autophagy by activating mTOR in HEK293, HeLa, and SH-SY5Y cells. Further studies indicated that miR-4465 reduced PTEN mRNA levels through posttranscriptional regulation via directly targeting the 3-UTR. Our novel findings provide useful hints for the comprehensive elucidation of the molecular mechanism of miRNA-regulated PTEN-related autophagy and may also provide some new insights for the exploration of miRNAs in the treatment of PTEN-related diseases.
关键词	Autophagy miR-4465 PTEN mTOR
收录类别	SCI ; SCIE
语种	英语
资助项目	Natural Science Foundation of Jiangsu Province[BK20150290]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000457837800010
出版者	SPRINGER
WOS关键词	CELL LUNG-CANCER ; GENE ; METASTASIS ; RAPAMYCIN ; BREAST
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/30243
专题	生命科学与技术学院_博士生生命科学与技术学院_硕士生
通讯作者	Wang, Mei
作者单位	1.Soochow Univ, Childrens Hosp, Dept Pharm, 92 Zhongnan St, Suzhou, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Materia Med, State Key Lab Drug Res, Ctr Drug Safety Evaluat & Res, Shanghai, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 4.Soochow Univ, Jiangsu Key Lab Neuropsychiat Dis, Lab Mol Neuropathol, Suzhou 215021, Peoples R China 5.Soochow Univ, Coll Pharmaceut Sci, Suzhou 215021, Peoples R China
推荐引用方式 GB/T 7714	Tao, Zhouteng,Feng, Chenxi,Mao, Chenmei,et al. MiR-4465 directly targets PTEN to inhibit AKT/mTOR pathway-mediated autophagy[J]. CELL STRESS & CHAPERONES,2019,24(1):105-113.
APA	Tao, Zhouteng.,Feng, Chenxi.,Mao, Chenmei.,Ren, Jin.,Tai, Yusi.,...&Wang, Mei.(2019).MiR-4465 directly targets PTEN to inhibit AKT/mTOR pathway-mediated autophagy.CELL STRESS & CHAPERONES,24(1),105-113.
MLA	Tao, Zhouteng,et al."MiR-4465 directly targets PTEN to inhibit AKT/mTOR pathway-mediated autophagy".CELL STRESS & CHAPERONES 24.1(2019):105-113.