Architecture, substructures, and dynamic assembly of STRIPAK complexes in Hippo signaling

doi:10.1038/s41421-018-0077-3

	Architecture, substructures, and dynamic assembly of STRIPAK complexes in Hippo signaling
	Tang, Yang 1; Chen, Min 1; Zhou, Li2 ; Ma, Jian 1; Li, Yehua 1; Zhang, Hui 1; Shi, Zhubing 1; Xu, Qi 1; Zhang, Xiaoman 1; Gao, Ziyang 1; Zhao, Yun1,2 ; Cheng, Yunfeng 3,4; Jiao, Shi 1; Zhou, Zhaocai1,2
	2019-01-08
发表期刊	CELL DISCOVERY
ISSN	2056-5968
卷号	5
发表状态	已发表
DOI	10.1038/s41421-018-0077-3
摘要	Striatin-interacting phosphatases and kinases (STRIPAKs) are evolutionarily conserved supramolecular complexes, which have been implicated in the Hippo signaling pathway. Yet the topological structure and dynamic assembly of STRIPAK complexes remain elusive. Here, we report the overall architecture and substructures of a Hippo kinase- containing STRIPAK complex. PP2Aa/c-bound STRN3 directly contacts the Hippo kinase MST2 and also controls the loading of MST2 via two "arms" in a phosphorylation-dependent manner, one arm being STRIP1 and the other SIKE1-SLMAP. A decreased cell density triggered the dissociation of the STRIP1 arm from STRIPAK, reflecting the dynamic assembly of the complex upon sensing upstream signals. Crystallographic studies defined at atomic resolution the interface between STRN3 and SIKE1, and that between SIKE1 and SLMAP. Disrupting the complex assembly abrogated the regulatory effect of STRIPAK towards Hippo signaling. Collectively, our study revealed a "two-arm" assembly of STRIPAK with context-dependent dynamics, offering a framework for further studies on Hippo signaling and biological processes involving MST kinases.
收录类别	SCI ; SCIE
语种	英语
资助项目	Strategic Priority Research Program[XDB19020202]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000454987900002
出版者	NATURE PUBLISHING GROUP
WOS关键词	PROTEIN PHOSPHATASE 2A ; STRIATIN-INTERACTING PHOSPHATASE ; CALMODULIN-BINDING PROTEIN ; CRYSTAL-STRUCTURE ; KINASE STRIPAK ; COILED-COIL ; CCP4 SUITE ; FHA DOMAIN ; ACTIVATION ; PATHWAY
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/30060
专题	生命科学与技术学院_硕士生生命科学与技术学院_特聘教授组_赵允组生命科学与技术学院_特聘教授组_周兆才组
通讯作者	Jiao, Shi; Zhou, Zhaocai
作者单位	1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci,State Key Lab Cel, Shanghai 200031, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 3.Fudan Univ, Zhongshan Hosp, Dept Hematol, Shanghai 200032, Peoples R China 4.Fudan Univ, Zhongshan Hosp, Inst Clin Sci, Shanghai 200032, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Tang, Yang,Chen, Min,Zhou, Li,et al. Architecture, substructures, and dynamic assembly of STRIPAK complexes in Hippo signaling[J]. CELL DISCOVERY,2019,5.
APA	Tang, Yang.,Chen, Min.,Zhou, Li.,Ma, Jian.,Li, Yehua.,...&Zhou, Zhaocai.(2019).Architecture, substructures, and dynamic assembly of STRIPAK complexes in Hippo signaling.CELL DISCOVERY,5.
MLA	Tang, Yang,et al."Architecture, substructures, and dynamic assembly of STRIPAK complexes in Hippo signaling".CELL DISCOVERY 5(2019).