Programmable and printable Bacillus subtilis biofilms as engineered living materials

doi:10.1038/s41589-018-0169-2

	Programmable and printable Bacillus subtilis biofilms as engineered living materials
	Huang, Jiaofang1 ; Liu, Suying1,2,3 ; Zhang, Chen1,4 ; Wang, Xinyu1 ; Pu, Jiahua1 ; Ba, Fang5 ; Xue, Shuai 6,7; Ye, Haifeng 6,7; Zhao, Tianxin1 ; Li, Ke1 ; Wang, Yanyi1 ; Zhang, Jicong1 ; Wang, Lihua 2,3,8; Fan, Chunhai 2,3,9,10; Lu, Timothy K.11; Zhong, Chao1
	2019-01
发表期刊	NATURE CHEMICAL BIOLOGY (IF:12.9[JCR-2023],14.7[5-Year])
ISSN	1552-4450
卷号	15 期号:1 页码:34-+
发表状态	已发表
DOI	10.1038/s41589-018-0169-2
摘要	Bacterial biofilms can be programmed to produce living materials with self-healing and evolvable functionalities. However, the wider use of artificial biofilms has been hindered by limitations on processability and functional protein secretion capacity. We describe a highly flexible and tunable living functional materials platform based on the TasA amyloid machinery of the bacterium Bacillus subtilis. We demonstrate that genetically programmable TasA fusion proteins harboring diverse functional proteins or domains can be secreted and can assemble into diverse extracellular nano-architectures with tunable physicochemical properties. Our engineered biofilms have the viscoelastic behaviors of hydrogels and can be precisely fabricated into microstructures having a diversity of three-dimensional (3D) shapes using 3D printing and microencapsulation techniques. Notably, these long-lasting and environmentally responsive fabricated living materials remain alive, self-regenerative, and functional. This new tunable platform offers previously unattainable properties for a variety of living functional materials having potential applications in biomaterials, biotechnology, and biomedicine.
收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China (NSFC)[31522017] ; National Natural Science Foundation of China (NSFC)[31470834] ; National Natural Science Foundation of China (NSFC)[31670869]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000452634100013
出版者	NATURE PUBLISHING GROUP
WOS关键词	PROTEIN ; STRATEGIES ; INSIGHTS ; STRAIN ; MATRIX ; FIBER
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/29979
专题	生命科学与技术学院_本科生物质科学与技术学院_PI研究组_钟超组生命科学与技术学院_硕士生物质科学与技术学院_硕士生物质科学与技术学院_博士生
通讯作者	Zhong, Chao
作者单位	1.ShanghaiTech Univ, Mat & Phys Biol Div, Sch Phys Sci & Technol, Shanghai, Peoples R China 2.Chinese Acad Sci, Div Phys Biol, Shanghai Inst Appl Phys, Shanghai, Peoples R China 3.Chinese Acad Sci, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil, Shanghai Inst Appl Phys,CAS Key Lab Interfacial P, Shanghai, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai, Peoples R China 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 6.East China Normal Univ, Shanghai Key Lab Regulatory Biol, Inst Biomed Sci, Shanghai, Peoples R China 7.East China Normal Univ, Sch Life Sci, Shanghai, Peoples R China 8.EastChina Normal Univ, Shanghai Key Lab Green Chem & Chem Proc, Sch Chem & Mol Engn, Shanghai, Peoples R China 9.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Sch Med, Renji Hosp, Shanghai, Peoples R China 10.Shanghai Jiao Tong Univ, Inst Mol Med, Sch Med, Renji Hosp, Shanghai, Peoples R China 11.MIT, Synthet Biol Grp, Res Lab Elect, 77 Massachusetts Ave, Cambridge, MA 02139 USA
第一作者单位	物质科学与技术学院
通讯作者单位	物质科学与技术学院
第一作者的第一单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Huang, Jiaofang,Liu, Suying,Zhang, Chen,et al. Programmable and printable Bacillus subtilis biofilms as engineered living materials[J]. NATURE CHEMICAL BIOLOGY,2019,15(1):34-+.
APA	Huang, Jiaofang.,Liu, Suying.,Zhang, Chen.,Wang, Xinyu.,Pu, Jiahua.,...&Zhong, Chao.(2019).Programmable and printable Bacillus subtilis biofilms as engineered living materials.NATURE CHEMICAL BIOLOGY,15(1),34-+.
MLA	Huang, Jiaofang,et al."Programmable and printable Bacillus subtilis biofilms as engineered living materials".NATURE CHEMICAL BIOLOGY 15.1(2019):34-+.