特异性抑制连接蛋白26的全人抗体

	特异性抑制连接蛋白26的全人抗体
翻译题名	A FULLY HUMAN ANTIBODY SPECIFICALLY INHIBITING CONNEXIN 26
申请号	EP20196319
	2021-02-17
公开（公告）号	EP3778633A1
公开日期	2021-02-17
摘要	本发明提供了一种特异性抑制连接蛋白26的全人抗体，其特征在于它是一种具有scFv-Fc结构的重组免疫球蛋白，其中scFv是指包含重链可变区和轻链可变区的单链抗体，重链可变区的氨基酸序列为SEQ ID NO : 1，轻链可变区的氨基酸序列为SEQ ID NO : 2，Fc为恒定区。本发明以连接蛋白26胞外区的第一个41-56个氨基酸序列为抗原，其序列为KEVWGDEQADFVCNTL。通过对单链抗体噬菌体展示文库获得的抗体进行生化分析和免疫荧光鉴定及筛选技术，证实本发明的抗体能够特异性识别连接蛋白26并抑制其半通道活性。动物实验表明，该抗体显著抑制小鼠耳蜗组织切片的半通道活性。因此，该抗体可用于连接蛋白突变相关疾病的治疗。
翻译摘要	The present invention provides a fully human antibody specifically inhibiting Connexin 26, characterized in that it is a recombinant immunoglobulin having the structure of scFv-Fc, wherein scFv refers to a single-chain antibody comprising a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is SEQ ID NO : 1, the amino acid sequence of the light chain variable region is SEQ ID NO : 2, and Fc refers to constant region. The present invention uses the first 41-56 amino acid sequence of extracellular region of Connexin26 as an antigen, the sequence thereof is KEVWGDEQADFVCNTL. Through the biochemical analysis and immunofluorescence identification of the antibody obtained by single-chain antibody phage display library and screening technology, it was confirmed that the antibody provided by the present invention could specifically recognize Connexin 26 and inhibit its hemichannel activity. Animal experiments show that this antibody significantly inhibits the hemichannel activity of mouse cochlear tissue sections. Therefore, the antibody can be used in the treatment of Connexin mutation associated diseases.
当前权利人	Shanghaitech University
专利代理人	Cabinet Beau De Loménie
代理机构	Cabinet Beau De Loménie
专利申请人	Shanghaitech University
	Qu Zhihu; Yang Guang; Mammano Fabio; Zonta Francesco
公开国别	欧洲专利局
公开国别简称	EP
IPC 分类号	C07K16//18; A61K39//395; C12N15//11
CPC分类号	C07K16//18; A61K2039//505; C07K2317//524; C07K2317//526; C07K2317//622; C07K2317//21; C07K2317//76; C07K2317//94; C07K2317//92; C07K2317//51; C07K2317//55; C07K2317//565
专利有效性	失效
专利类型	发明申请
专利类型字典	1
当前法律状态	授权
简单同族	JP7022690B2; EP3778633A1; US11345746B2; ES2824176T3; EP3342784A4; WO2017128880A1; CA3041744A1; CN105566495B; CN105566495A; EP3342784B1; JP2019504829A; EP3342784A1; US20200299366A1
扩展同族	EP3342784A4; JP7022690B2; US20200299366A1; CA3041744A1; CN105566495A; ES2824176T3; JP2019504829A; EP3342784B1; WO2017128880A1; CN105566495B; US11345746B2; EP3778633A1; EP3342784A1; JP2019504829A5
INPADOC 同族	EP3342784A1; ES2824176T3; US20200299366A1; WO2017128880A1; JP7022690B2; EP3342784B1; CA3041744A1; CN105566495B; EP3342784A4; US11345746B2; CN105566495A; JP2019504829A; EP3778633A1
文献类型	专利
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/294820
专题	免疫化学研究所_特聘教授组_功能筛选实验室免疫化学研究所_特聘教授组_Michael Levitt组免疫化学研究所_公共科研平台_高通量筛选平台
作者单位	Shanghaitech University
推荐引用方式 GB/T 7714	Qu Zhihu,Yang Guang,Mammano Fabio,等. 特异性抑制连接蛋白26的全人抗体. EP20196319[P]. 2021-02-17.