Electrospun nanofiber regulates assembly of keratin and vimentin intermediate filaments of PANC-1 pancreatic carcinoma cells
Wang, Yiqun1,2; Lu, Yi1; Gong, Jinkang1; Yao, Yuan1
2019-03
Source PublicationMATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
ISSN0928-4931
Volume96Pages:616-624
AbstractIntermediate filaments, together with actin microfilaments and microtubules constituent the cytoskeleton of mammalian cells, involving in various cellular activities. The roles of intermediate filaments in cell skeleton reorganization when responding with extracellular matrix (ECM) nanostructure are poorly understood yet. To unveil the effects of fibrous composition and orientation on cells, we developed electrospun nanofibers of varying topology and components, and the effects on assembly of intermediate filaments as keratin and vimentin were investigated in detail. We found that aligned nanofibers enhanced expression of E-cadherin and promoted assembly of keratin intermediate filaments. Meanwhile, the compositional variation show different preference on up-regulation of the two intermediate filaments. Compared to keratin, the assembly of vimentin intermediate filaments were promoted by incorporating bovine serum albumin (BSA) functionalized graphene oxide (BSA-GO) into polycaprolactone (PCL) nanofibers. Thus, our findings elucidate how the different physical factors of fibrous extracellular matrix affect the reorganization of cytoskeleton by assembly of keratin and vimentin intermediate filaments.
KeywordElectrospun nanofibers Intermediate filaments Aligned nanofibers Keratin Vimentin
DOI10.1016/j.msec.2018.11.072
Indexed ByEI ; SCI
Language英语
WOS Research AreaMaterials Science
WOS SubjectMaterials Science, Biomaterials
WOS IDWOS:000456760700064
PublisherELSEVIER SCIENCE BV
EI Accession Number20184906211055
EI KeywordsBody fluids
EI Classification NumberBiological Materials and Tissue Engineering:461.2 ; Nanotechnology:761 ; Chemical Products Generally:804 ; Organic Compounds:804.1 ; Solid State Physics:933
WOS KeywordMODIFIED GRAPHENE OXIDE ; OSTEOGENIC DIFFERENTIATION ; CANCER-CELLS ; STEM-CELLS ; IN-VITRO ; MIGRATION ; INVASION ; ADHESION ; TUMOR ; DELIVERY
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Document Type期刊论文
Identifierhttp://kms.shanghaitech.edu.cn/handle/2MSLDSTB/29097
Collection物质科学与技术学院_PI研究组_龚晋慷组
物质科学与技术学院_硕士生
Corresponding AuthorGong, Jinkang; Yao, Yuan
Affiliation1.Shanghai Tech Univ, Sch Phys Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Organ Chem, 345 Lingling Rd, Shanghai 200032, Peoples R China
First Author AffilicationSchool of Physical Science and Technology
Corresponding Author AffilicationSchool of Physical Science and Technology
First Signature AffilicationSchool of Physical Science and Technology
Recommended Citation
GB/T 7714
Wang, Yiqun,Lu, Yi,Gong, Jinkang,et al. Electrospun nanofiber regulates assembly of keratin and vimentin intermediate filaments of PANC-1 pancreatic carcinoma cells[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2019,96:616-624.
APA Wang, Yiqun,Lu, Yi,Gong, Jinkang,&Yao, Yuan.(2019).Electrospun nanofiber regulates assembly of keratin and vimentin intermediate filaments of PANC-1 pancreatic carcinoma cells.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,96,616-624.
MLA Wang, Yiqun,et al."Electrospun nanofiber regulates assembly of keratin and vimentin intermediate filaments of PANC-1 pancreatic carcinoma cells".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 96(2019):616-624.
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