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Electrospun nanofiber regulates assembly of keratin and vimentin intermediate filaments of PANC-1 pancreatic carcinoma cells | |
Wang, Yiqun1,2; Lu, Yi1; Gong, Jinkang1; Yao, Yuan1 | |
2019-03 | |
发表期刊 | MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS |
ISSN | 0928-4931 |
卷号 | 96页码:616-624 |
发表状态 | 已发表 |
DOI | 10.1016/j.msec.2018.11.072 |
摘要 | Intermediate filaments, together with actin microfilaments and microtubules constituent the cytoskeleton of mammalian cells, involving in various cellular activities. The roles of intermediate filaments in cell skeleton reorganization when responding with extracellular matrix (ECM) nanostructure are poorly understood yet. To unveil the effects of fibrous composition and orientation on cells, we developed electrospun nanofibers of varying topology and components, and the effects on assembly of intermediate filaments as keratin and vimentin were investigated in detail. We found that aligned nanofibers enhanced expression of E-cadherin and promoted assembly of keratin intermediate filaments. Meanwhile, the compositional variation show different preference on up-regulation of the two intermediate filaments. Compared to keratin, the assembly of vimentin intermediate filaments were promoted by incorporating bovine serum albumin (BSA) functionalized graphene oxide (BSA-GO) into polycaprolactone (PCL) nanofibers. Thus, our findings elucidate how the different physical factors of fibrous extracellular matrix affect the reorganization of cytoskeleton by assembly of keratin and vimentin intermediate filaments. |
关键词 | Electrospun nanofibers Intermediate filaments Aligned nanofibers Keratin Vimentin |
收录类别 | EI ; SCIE ; SCI |
语种 | 英语 |
WOS研究方向 | Materials Science |
WOS类目 | Materials Science, Biomaterials |
WOS记录号 | WOS:000456760700064 |
出版者 | ELSEVIER SCIENCE BV |
EI入藏号 | 20184906211055 |
EI主题词 | Body fluids ; Cells ; Cytology ; Graphene ; Keratin ; Mammals |
EI分类号 | Biological Materials and Tissue Engineering:461.2 ; Nanotechnology:761 ; Chemical Products Generally:804 ; Organic Compounds:804.1 ; Solid State Physics:933 |
WOS关键词 | MODIFIED GRAPHENE OXIDE ; OSTEOGENIC DIFFERENTIATION ; CANCER-CELLS ; STEM-CELLS ; IN-VITRO ; MIGRATION ; INVASION ; ADHESION ; TUMOR ; DELIVERY |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/29097 |
专题 | 物质科学与技术学院_硕士生 物质科学与技术学院_PI研究组_龚晋慷组 附属学校、幼儿园筹建部 |
通讯作者 | Gong, Jinkang; Yao, Yuan |
作者单位 | 1.Shanghai Tech Univ, Sch Phys Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Organ Chem, 345 Lingling Rd, Shanghai 200032, Peoples R China |
第一作者单位 | 物质科学与技术学院 |
通讯作者单位 | 物质科学与技术学院 |
第一作者的第一单位 | 物质科学与技术学院 |
推荐引用方式 GB/T 7714 | Wang, Yiqun,Lu, Yi,Gong, Jinkang,et al. Electrospun nanofiber regulates assembly of keratin and vimentin intermediate filaments of PANC-1 pancreatic carcinoma cells[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2019,96:616-624. |
APA | Wang, Yiqun,Lu, Yi,Gong, Jinkang,&Yao, Yuan.(2019).Electrospun nanofiber regulates assembly of keratin and vimentin intermediate filaments of PANC-1 pancreatic carcinoma cells.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,96,616-624. |
MLA | Wang, Yiqun,et al."Electrospun nanofiber regulates assembly of keratin and vimentin intermediate filaments of PANC-1 pancreatic carcinoma cells".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 96(2019):616-624. |
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