Photocrosslinked hydrogels from coumarin derivatives of hyaluronic acid for tissue engineering applications

doi:10.1016/j.msec.2018.11.052

KMS > 免疫化学研究所 > 特聘教授组 > Nicola Elvassore组

	Photocrosslinked hydrogels from coumarin derivatives of hyaluronic acid for tissue engineering applications
	Beninatto, Riccardo 1; Barbera, Carlo 1; De Lucchi, Ottorino 2; Borsato, Giuseppe 2; Serena, Elena 3,4; Guarise, Cristian 1; Pavan, Mauro 1; Luni, Camilla5 ; Martewicz, Sebastian5 ; Galesso, Devis 1; Elvassore, Nicola3,4,5
	2019-03
发表期刊	MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS (IF:8.1[JCR-2023],7.5[5-Year])
ISSN	0928-4931
卷号	96 页码:625-634
发表状态	已发表
DOI	10.1016/j.msec.2018.11.052
摘要	Hydrogels are an increasingly attractive choice in the fields of regenerative medicine, wound care and tissue engineering as important forms of bio-scaffolds. For many clinical needs, injectable in situ crosslinkable hydrogels are strongly preferred, due to treatment effectiveness and ease of use. In this study, hyaluronic acid (HA), containing side-arms linked to photo-active coumarin moieties, was used for the preparation of wall-to-wall hydrogels. This photocrosslinkable HA, hereafter called HA-TEG-coumarin, produces colourless aqueous solutions that solidify upon near-UV irradiation (at a specific wavelength of 365 nm) via a clean [2 + 2] photocycloaddition reaction, without by-products formation. The crosslinking event, a robust and non-cytotoxic process, does not require catalysts or radical initiators: in the field of hyaluronan photocrosslinking, this innovative feature is significant to ensure the whole biocompatibility and to avoid collateral reactions. Mechanical and rheological tests showed that hyaluronan derivatives became hydrogels after 3-5 min of irradiation, with average values for bulk and surface elastic moduli of about 32 kPa and 193 kPa, respectively. Fluorescence recovery after photobleaching (FRAP) assay showed that the hydrogels are porous and allow a good permeation for nutrients and growth factors. Cell metabolism and proliferation assays revealed that hydrogel-encapsulated fibroblasts maintained their viability and that HA-TEG-coumarin sustained the proliferation of non-adherent myoblasts. For all of these reasons and thanks to a safe free-radical approach, this novel hyaluronan coumarin derivative could be a good candidate for tissue engineering and regenerative medicine applications.
关键词	Hyaluronic acid Hydrogel Scaffold Coumarin Photocrosslinking Tissue engineering
收录类别	EI ; SCIE ; SCI
语种	英语
WOS研究方向	Materials Science
WOS类目	Materials Science, Biomaterials
WOS记录号	WOS:000456760700065
出版者	ELSEVIER SCIENCE BV
EI入藏号	20184906211353
EI主题词	Biocompatibility ; Cell culture ; Crosslinking ; Free radicals ; Hydrogels ; Irradiation ; Metabolism ; Organic acids ; Photobleaching ; Regenerative Medicine ; Scaffolds ; Scaffolds (biology) ; Tissue ; Tissue engineering
EI分类号	Construction Equipment:405.1 ; Bioengineering and Biology:461 ; Chemistry:801 ; Chemical Reactions:802.2 ; Chemical Products Generally:804 ; Organic Compounds:804.1
WOS关键词	SKELETAL-MUSCLE ; PHOTODIMERIZATION ; REPAIR
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/29096
专题	免疫化学研究所_特聘教授组_生物工程学实验室
通讯作者	Beninatto, Riccardo; Elvassore, Nicola
作者单位	1.Fidia Farmaceutici SpA, Via Ponte Fabbr 3-A, I-35031 Abano Terme, Italy 2.Univ Ca Foscari Venezia, Dipartimento Sci Mol & Nanosistemi, Venice, Italy 3.Univ Padua, Dipartimento Ingn Ind, Padua, Italy 4.VIMM, Padua, Italy 5.ShanghaiTech Univ, SIAIS, Shanghai, Peoples R China
通讯作者单位	上海科技大学
推荐引用方式 GB/T 7714	Beninatto, Riccardo,Barbera, Carlo,De Lucchi, Ottorino,et al. Photocrosslinked hydrogels from coumarin derivatives of hyaluronic acid for tissue engineering applications[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2019,96:625-634.
APA	Beninatto, Riccardo.,Barbera, Carlo.,De Lucchi, Ottorino.,Borsato, Giuseppe.,Serena, Elena.,...&Elvassore, Nicola.(2019).Photocrosslinked hydrogels from coumarin derivatives of hyaluronic acid for tissue engineering applications.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,96,625-634.
MLA	Beninatto, Riccardo,et al."Photocrosslinked hydrogels from coumarin derivatives of hyaluronic acid for tissue engineering applications".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 96(2019):625-634.