Making human cardiomyocytes up to date: Derivation, maturation state and perspectives

doi:10.1016/j.ijcard.2017.03.099

	Making human cardiomyocytes up to date: Derivation, maturation state and perspectives
	Kolanowski, Tomasz J.1; Antos, Christopher L.1,2 ; Guan, Kaomei 1
	2017-08-15
发表期刊	INTERNATIONAL JOURNAL OF CARDIOLOGY (IF:3.2[JCR-2023],3.2[5-Year])
ISSN	0167-5273
卷号	241 页码:379-386
发表状态	已发表
DOI	10.1016/j.ijcard.2017.03.099
摘要	In vitro generation of cardiomyocytes (CMs) from human cells opens the possibility to develop patient-specific therapies to various cardiomyopathies. By establishing the in vitro reprograming methods that produce human CMs, we learn about what is involved in the development of specific CM subtypes. In this review, we summarize the latest achievements in CM generation technologies, emphasizing the differentiation methods of specific CM subtypes. We also relate the biological properties and functions of the in vitro-generated CMs to those of their in vivo counterparts. Furthermore, we describe the main problem of current CM derivation methods - maturation of CMs. We subsequently discuss biochemical and physical stimuli that are used to overcome the maturation problems of in vitro-derived CMs. As a result, amore holistic approach with controllable environment and timing of specific stimuli for creation of more mature engineered heart tissues is described as well. Finally, we propose a novel approach in which enhancing energy transfer mechanisms in the immature CMs might help to overcome the current hurdle of incomplete in vitro differentiation. (C) 2017 The Authors. Published by Elsevier Ireland Ltd.
关键词	Stem cells Induced pluripotent stem cells Cardiomyocyte derivation protocols Cardiomyocyte maturation In vitro derived-cardiomyocyte applications
收录类别	SCI
语种	英语
资助项目	DFG[AN 797/4-1]
WOS研究方向	Cardiovascular System & Cardiology
WOS类目	Cardiac & Cardiovascular Systems
WOS记录号	WOS:000405455200065
出版者	ELSEVIER IRELAND LTD
WOS关键词	PLURIPOTENT STEM-CELLS ; IN-VITRO ; ELECTRICAL-STIMULATION ; ENGINEERED MYOCARDIUM ; DIFFERENTIATION ; FIBROBLASTS ; SYSTEM ; CONTRACTION ; MODULATION ; ACTIVATION
原始文献类型	Article
通讯作者	Guan, Kaomei
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2868
专题	生命科学与技术学院生命科学与技术学院_PI研究组_Christopher Lee Antos组
通讯作者	Guan, Kaomei
作者单位	1.Tech Univ Dresden, Inst Pharmacol & Toxicol, Fetscherstr 74, D-01307 Dresden, Germany 2.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Kolanowski, Tomasz J.,Antos, Christopher L.,Guan, Kaomei. Making human cardiomyocytes up to date: Derivation, maturation state and perspectives[J]. INTERNATIONAL JOURNAL OF CARDIOLOGY,2017,241:379-386.
APA	Kolanowski, Tomasz J.,Antos, Christopher L.,&Guan, Kaomei.(2017).Making human cardiomyocytes up to date: Derivation, maturation state and perspectives.INTERNATIONAL JOURNAL OF CARDIOLOGY,241,379-386.
MLA	Kolanowski, Tomasz J.,et al."Making human cardiomyocytes up to date: Derivation, maturation state and perspectives".INTERNATIONAL JOURNAL OF CARDIOLOGY 241(2017):379-386.