The Key Roles of Mycobacterium tuberculosis FadD23 C-terminal Domain in Catalytic Mechanisms

doi:10.3389/fmicb.2023.1090534

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	The Key Roles of Mycobacterium tuberculosis FadD23 C-terminal Domain in Catalytic Mechanisms
	Yan, Mengrong 1; Cao, Lin 1; Zhao, Li 2; Zhou, Weihong 1; Liu, Xiang 1; Zhang, Wei 2; Rao, Zihe1,2,3,4,5,6
	2023
发表期刊	FRONTIERS IN MICROBIOLOGY; (IF:4.0[JCR-2023],5.1[5-Year])
ISSN	1664-302X
EISSN	1664-302X
卷号	14
发表状态	已发表
DOI	10.3389/fmicb.2023.1090534
摘要	Sulfolipid-1 (SL-1) is located in the Mycobacterium tuberculosis (M. tb) cell wall, and is essential for pathogen virulence and intracellular growth. Multiple proteins (e.g., Pks2, FadD23, PapA1, and MmpL8) in the SL-1 synthesis pathway can be treated as drug targets, but, to date, their structures have not been solved. The crystal structures of FadD23 bound to ATP or hexadecanoyl adenylate was determined in this study. We have also investigated long-chain saturated fatty acids as biological substrates of FadD23 through structural, biological, and chemical analyses. The mutation at the active site of FadD23 greatly influences enzymatic activity. Meanwhile, the FadD23 N-terminal domain alone cannot bind palmitic acid without C-terminal domain facilitation since it is almost inactive after removing the C-terminal domain. FadD23 is the first protein in the SL-1 synthesis pathway whose structure has been solved. These results reveal the importance of the C-terminal domain in the catalytic mechanism.
关键词	catalytic mechanism crystal structure enzymatic activity fatty acid adenylating enzyme sulfolipid-1
学科门类	Microbiology ; Microbiology (medical)
URL	查看原文
收录类别	SCOPUS ; SCI
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000943621500001
出版者	FRONTIERS MEDIA SA
原始文献类型	Article
Scopus 记录号	2-s2.0-85149638075
来源库	SCOPUS
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/286507
专题	免疫化学研究所生命科学与技术学院免疫化学研究所_特聘教授组_饶子和组
通讯作者	Liu, Xiang; Zhang, Wei; Rao, Zihe
作者单位	1.Nankai Univ, Coll Life Sci, Frontiers Sci Ctr Cell Responses, State Key Lab Med Chem Biol, Tianjin, Peoples R China 2.Guangzhou Lab, Innovat Ctr Pathogen Res, Guangzhou, Peoples R China 3.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 5.Tsinghua Univ, Sch Life Sci, Lab Struct Biol, Beijing, Peoples R China 6.Tsinghua Univ, Sch Med, Beijing, Peoples R China
通讯作者单位	免疫化学研究所; 生命科学与技术学院
推荐引用方式 GB/T 7714	Yan, Mengrong,Cao, Lin,Zhao, Li,et al. The Key Roles of Mycobacterium tuberculosis FadD23 C-terminal Domain in Catalytic Mechanisms[J]. FRONTIERS IN MICROBIOLOGY;,2023,14.
APA	Yan, Mengrong.,Cao, Lin.,Zhao, Li.,Zhou, Weihong.,Liu, Xiang.,...&Rao, Zihe.(2023).The Key Roles of Mycobacterium tuberculosis FadD23 C-terminal Domain in Catalytic Mechanisms.FRONTIERS IN MICROBIOLOGY;,14.
MLA	Yan, Mengrong,et al."The Key Roles of Mycobacterium tuberculosis FadD23 C-terminal Domain in Catalytic Mechanisms".FRONTIERS IN MICROBIOLOGY; 14(2023).