APC/C-dependent degradation of Spd2 regulates centrosome asymmetry in Drosophila neural stem cells

doi:10.15252/embr.202255607

	APC/C-dependent degradation of Spd2 regulates centrosome asymmetry in Drosophila neural stem cells
	Meghini, Francesco 1; Martins, Torcato 1,2; Zhang, Qian3 ; Loyer, Nicolas 4; Trickey, Michelle 5; Abula, Yusanjiang 3; Yamano, Hiroyuki 5; Januschke, Jens 4; Kimata, Yuu1,3
	2023
发表期刊	EMBO REPORTS; (IF:6.5[JCR-2023],8.3[5-Year])
ISSN	1469-221X
EISSN	1469-3178
DOI	10.15252/embr.202255607
摘要	A functional centrosome is vital for the development and physiology of animals. Among numerous regulatory mechanisms of the centrosome, ubiquitin-mediated proteolysis is known to be critical for the precise regulation of centriole duplication. However, its significance beyond centrosome copy number control remains unclear. Using an in vitro screen for centrosomal substrates of the APC/C ubiquitin ligase in Drosophila, we identify several conserved pericentriolar material (PCM) components, including the inner PCM protein Spd2. We show that Spd2 levels are controlled by the interphase-specific form of APC/C, APC/CFzr, in cultured cells and developing brains. Increased Spd2 levels compromise neural stem cell–specific asymmetric PCM recruitment and microtubule nucleation at interphase centrosomes, resulting in partial randomisation of the division axis and segregation patterns of the daughter centrosome in the following mitosis. We further provide evidence that APC/CFzr-dependent Spd2 degradation restricts the amount and mobility of Spd2 at the daughter centrosome, thereby facilitating the accumulation of Polo-dependent Spd2 phosphorylation for PCM recruitment. Our study underpins the critical role of cell cycle–dependent proteolytic regulation of the PCM in stem cells.
关键词	asymmetric cell division cell cycle centrosome stem cell ubiquitination
学科门类	Biochemistry ; Molecular Biology ; Genetics
URL	查看原文
收录类别	SCOPUS
语种	英语
原始文献类型	Article
Scopus 记录号	2-s2.0-85149273769
来源库	SCOPUS
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/286497
专题	生命科学与技术学院生命科学与技术学院_博士生生命科学与技术学院_PI研究组_Yuu Kimita组
作者单位	1.Department of Genetics,University of Cambridge,Cambridge,United Kingdom; 2.Department of Medical Sciences,Institute of Biomedicine-iBiMED,University of Aveiro,Aveiro,Portugal; 3.School of Life Science and Technology,ShanghaiTech University,Shanghai,China; 4.School of Life Science,University of Dundee,Dundee,United Kingdom; 5.UCL Cancer Institute,University College London,London,United Kingdom
推荐引用方式 GB/T 7714	Meghini, Francesco,Martins, Torcato,Zhang, Qian,et al. APC/C-dependent degradation of Spd2 regulates centrosome asymmetry in Drosophila neural stem cells[J]. EMBO REPORTS;,2023.
APA	Meghini, Francesco.,Martins, Torcato.,Zhang, Qian.,Loyer, Nicolas.,Trickey, Michelle.,...&Kimata, Yuu.(2023).APC/C-dependent degradation of Spd2 regulates centrosome asymmetry in Drosophila neural stem cells.EMBO REPORTS;.
MLA	Meghini, Francesco,et al."APC/C-dependent degradation of Spd2 regulates centrosome asymmetry in Drosophila neural stem cells".EMBO REPORTS; (2023).