Chromosome territory reorganization through artificial chromosome fusion is compatible with cell fate determination and mouse development

doi:10.1038/s41421-022-00511-1

	Chromosome territory reorganization through artificial chromosome fusion is compatible with cell fate determination and mouse development
	Wang, Yuang 1; Qu, Zhen 2; Fang, Yi 2; Chen, Yulong 2; Peng, Jiayin 1; Song, Jiawen 1; Li, Jinsong1,3,4 ; Shi, Jiantao 2; Zhou, Jin-Qiu2,3,4 ; Zhao, Yun1,3,4
	2023-12-01
发表期刊	CELL DISCOVERY; (IF:13.0[JCR-2023],14.8[5-Year])
ISSN	2056-5968
EISSN	2056-5968
卷号	9 期号:1
DOI	10.1038/s41421-022-00511-1
摘要	Chromosomes occupy discrete spaces in the interphase cell nucleus, called chromosome territory. The structural and functional relevance of chromosome territory remains elusive. We fused chromosome 15 and 17 in mouse haploid embryonic stem cells (haESCs), resulting in distinct changes of territories in the cognate chromosomes, but with little effect on gene expression, pluripotency and gamete functions of haESCs. The karyotype-engineered haESCs were successfully implemented in generating heterozygous (2n = 39) and homozygous (2n = 38) mouse models. Mice containing the fusion chromosome are fertile, and their representative tissues and organs display no phenotypic abnormalities, suggesting unscathed development. These results indicate that the mammalian chromosome architectures are highly resilient, and reorganization of chromosome territories can be readily tolerated during cell differentiation and mouse development.
学科门类	Biochemistry ; Molecular Biology ; Genetics ; Cell Biology
URL	查看原文
收录类别	SCOPUS
语种	英语
原始文献类型	Article
Scopus 记录号	2-s2.0-85146779794
来源库	SCOPUS
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/286450
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_赵允组生命科学与技术学院_特聘教授组_周金秋组生命科学与技术学院_特聘教授组_李劲松组
作者单位	1.State Key Laboratory of Cell Biology,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,University of Chinese Academy of Sciences,Shanghai,China; 2.State Key Laboratory of Molecular Biology,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,University of Chinese Academy of Sciences,Shanghai,China; 3.Key Laboratory of Systems Health Science of Zhejiang Province,School of Life Science,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou,China; 4.School of Life Science and Technology,ShanghaiTech University,Shanghai,China
推荐引用方式 GB/T 7714	Wang, Yuang,Qu, Zhen,Fang, Yi,et al. Chromosome territory reorganization through artificial chromosome fusion is compatible with cell fate determination and mouse development[J]. CELL DISCOVERY;,2023,9(1).
APA	Wang, Yuang.,Qu, Zhen.,Fang, Yi.,Chen, Yulong.,Peng, Jiayin.,...&Zhao, Yun.(2023).Chromosome territory reorganization through artificial chromosome fusion is compatible with cell fate determination and mouse development.CELL DISCOVERY;,9(1).
MLA	Wang, Yuang,et al."Chromosome territory reorganization through artificial chromosome fusion is compatible with cell fate determination and mouse development".CELL DISCOVERY; 9.1(2023).