Sensing of a spore surface protein by a Drosophila chemosensory protein induces behavioral defense against fungal parasitic infections

doi:10.1016/j.cub.2022.11.004

	Sensing of a spore surface protein by a Drosophila chemosensory protein induces behavioral defense against fungal parasitic infections
	Shang, Junmei 1,2; Tang, Guirong 1,2; Yang, Jun 2,3; Lu, Mengting 1,2; Wang, Chen-Zhu 2,3; Wang, Chengshu1,2,4
	2023-01-23
发表期刊	CURRENT BIOLOGY; (IF:8.1[JCR-2023],9.8[5-Year])
ISSN	0960-9822
EISSN	1879-0445
卷号	33 期号:2 页码:276-286.e5
DOI	10.1016/j.cub.2022.11.004
摘要	In addition to innate immunity in a physiological context, insects have evolved behavioral defenses against parasite attacks. Here, we report that Drosophila can sense the CFEM (common in fungal extracellular membrane) protein Mcdc9, which acts as a negative virulence factor of the entomopathogenic fungus Metarhizium robertsii. The individual deletions of 18 CFEM genes in Metarhizium followed by fly infection identified three null mutants that could kill the flies more quickly than the wild-type strain, among which Mcdc9 can coat fungal spores and interact with the fly chemosensory protein CheA75a. The deletion of Mcdc9 in the fungus or the knockdown of CheA75a in flies had a similar effect, in which a greater number of fungal spores were left on flies than on the respective controls after topical infection. Thus, similar to the accelerated death of the wild-type flies treated with ΔMcdc9, the CheA75aRNAi flies succumbed more quickly than the control insects topically challenged with the wild-type strain. The CheA75a gene is highly transcribed in fly legs and wings, and positive electrophysiological responses were evidenced in tarsal sensilla after stimulation with the Mcdc9 protein. The results imply that this CFEM protein could be sensed as a contact elicitor inducing the hygienic behavior of flies against fungal parasitic infection, which reveals a previously unsuspected mechanism of fungus-insect interactions.
关键词	behavioral defense CFEM protein chemosensory protein Drosophila grooming Metarhizium virulence
学科门类	Neuroscience (all) ; Biochemistry, Genetics and Molecular Biology (all) ; Agricultural and Biological Sciences (all)
URL	查看原文
收录类别	SCOPUS
语种	英语
原始文献类型	Article
Scopus 记录号	2-s2.0-85146332763
来源库	SCOPUS
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/286443
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_王成树组
作者单位	1.Key Laboratory of Insect Developmental and Evolutionary Biology,CAS Center for Excellence in Molecular Plant Sciences,Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai,200032,China; 2.CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing,100049,China; 3.State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing,100101,China; 4.School of Life Science and Technology,ShanghaiTech University,Shanghai,201210,China
推荐引用方式 GB/T 7714	Shang, Junmei,Tang, Guirong,Yang, Jun,et al. Sensing of a spore surface protein by a Drosophila chemosensory protein induces behavioral defense against fungal parasitic infections[J]. CURRENT BIOLOGY;,2023,33(2):276-286.e5.
APA	Shang, Junmei,Tang, Guirong,Yang, Jun,Lu, Mengting,Wang, Chen-Zhu,&Wang, Chengshu.(2023).Sensing of a spore surface protein by a Drosophila chemosensory protein induces behavioral defense against fungal parasitic infections.CURRENT BIOLOGY;,33(2),276-286.e5.
MLA	Shang, Junmei,et al."Sensing of a spore surface protein by a Drosophila chemosensory protein induces behavioral defense against fungal parasitic infections".CURRENT BIOLOGY; 33.2(2023):276-286.e5.