Use of a dual genetic system to decipher exocrine cell fate conversions in the adult pancreas

doi:10.1038/s41421-022-00485-0

	Use of a dual genetic system to decipher exocrine cell fate conversions in the adult pancreas
	Zhao, Huan 1; Huang, Xiuzhen 1; Liu, Zixin 1; Lai, Liang 2; Sun, Ruilin 2; Shen, Ruling 3; Li, Yan 1; He, Lingjuan 4; Pu, Wenjuan 1; Lv, Zan 1; Li, Yi 1; Han, Ximeng 1; Liu, Xiuxiu 1; Zhou, Bin1,5,6
	2023-12-01
发表期刊	CELL DISCOVERY; (IF:13.0[JCR-2023],14.8[5-Year])
ISSN	2056-5968
EISSN	2056-5968
卷号	9 期号:1
DOI	10.1038/s41421-022-00485-0
摘要	Unraveling cell fate plasticity during tissue homeostasis and repair can reveal actionable insights for stem cell biology and regenerative medicine. In the pancreas, it remains controversial whether lineage transdifferentiation among the exocrine cells occur under pathophysiological conditions. Here, to address this question, we used a dual recombinase-mediated genetic system that enables simultaneous tracing of pancreatic acinar and ductal cells using two distinct genetic reporters, avoiding the “ectopic” labeling by Cre-loxP recombination system. We found that acinar-to-ductal transdifferentiation occurs after pancreatic duct ligation or during caerulein-induced pancreatitis, but not during homeostasis or after partial pancreatectomy. On the other hand, pancreatic ductal cells contribute to new acinar cells after significant acinar cell loss. By genetic tracing of cell proliferation, we also quantify the cell proliferation dynamics and deduce the turnover rate of pancreatic exocrine lineages during homeostasis. Together, these results suggest that the lineage transdifferentiation happens between acinar cells and ductal cells in the pancreatic exocrine glands under specific conditions.
学科门类	Biochemistry ; Molecular Biology ; Genetics ; Cell Biology
URL	查看原文
收录类别	SCOPUS
语种	英语
原始文献类型	Article
Scopus 记录号	2-s2.0-85145593551
来源库	SCOPUS
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/286435
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_周斌组
作者单位	1.State Key Laboratory of Cell Biology,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,University of Chinese Academy of Sciences,Shanghai,China; 2.Shanghai Model Organisms Center,Inc.,Shanghai,China; 3.Shanghai Laboratory Animal Research Center,Shanghai,China; 4.School of Life Sciences,Westlake University,Zhejiang,Hangzhou,China; 5.School of Life Science and Technology,ShanghaiTech University,Shanghai,China; 6.Key Laboratory of Systems Health Science of Zhejiang Province,School of Life Science,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Zhejiang,Hangzhou,China
推荐引用方式 GB/T 7714	Zhao, Huan,Huang, Xiuzhen,Liu, Zixin,et al. Use of a dual genetic system to decipher exocrine cell fate conversions in the adult pancreas[J]. CELL DISCOVERY;,2023,9(1).
APA	Zhao, Huan.,Huang, Xiuzhen.,Liu, Zixin.,Lai, Liang.,Sun, Ruilin.,...&Zhou, Bin.(2023).Use of a dual genetic system to decipher exocrine cell fate conversions in the adult pancreas.CELL DISCOVERY;,9(1).
MLA	Zhao, Huan,et al."Use of a dual genetic system to decipher exocrine cell fate conversions in the adult pancreas".CELL DISCOVERY; 9.1(2023).