Genetic dissection of intercellular interactions in vivo by membrane-permeable protein

doi:10.1073/pnas.2120582120

	Genetic dissection of intercellular interactions in vivo by membrane-permeable protein
	Zhang, Shaohua 1; Zhang, Qianyu2 ; Liu, Zixin 1; Liu, Kuo 3; He, Lingjuan 4; Lui, Kathy O.5; Wang, Lixin 6; Zhou, Bin1,2,3
	2023-01-03
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA; (IF:9.4[JCR-2023],10.8[5-Year])
ISSN	0027-8424
EISSN	1091-6490
卷号	120 期号:1
DOI	10.1073/pnas.2120582120
摘要	Unraveling cell-cell interaction is fundamental to understanding many biological processes. To date, genetic tools for labeling neighboring cells in mammals are not available. Here, we developed a labeling strategy based on the Cre-induced intercellular labeling protein (CILP). Cre-expressing donor cells release a lipid-soluble and membrane-permeable fluorescent protein that is then taken up by recipient cells, enabling fluorescent labeling of neighboring cells. Using CILP, we specifically labeled endothelial cells surrounding a special population of hepatocytes in adult mice and revealed their distinct gene signatures. Our results highlight the potential of CILP as a platform to reveal cell-cell interactions and communications in vivo.
关键词	genetic tool intercellular interactions liver zonation membrane-permeable protein niche
学科门类	Multidisciplinary
URL	查看原文
收录类别	SCOPUS
语种	英语
原始文献类型	Article
Scopus 记录号	2-s2.0-85144803859
来源库	SCOPUS
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/286429
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_周斌组生命科学与技术学院_硕士生
作者单位	1.State Key Laboratory of Cell Biology,Center for Excellence in Molecular Cell Science,Shanghai Institute of Biochemistry and Cell Biology,Chinese Academy of Sciences,University of Chinese Academy of Sciences,Shanghai,200031,China; 2.School of Life Science and Technology,ShanghaiTech University,Shanghai,201210,China; 3.Key Laboratory of Systems Health Science of Zhejiang Province,School of Life Science,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou,310024,China; 4.School of Life Sciences,Westlake University,Hangzhou,210030,China; 5.Department of Chemical Pathology,Li Ka Shing Institute of Health Sciences,Prince of Wales Hospital,The Chinese University of Hong Kong,999077,Hong Kong; 6.Department of Cardiac Surgery,Zhongshan Hospital,Fudan University,Shanghai,200032,China
推荐引用方式 GB/T 7714	Zhang, Shaohua,Zhang, Qianyu,Liu, Zixin,et al. Genetic dissection of intercellular interactions in vivo by membrane-permeable protein[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA;,2023,120(1).
APA	Zhang, Shaohua.,Zhang, Qianyu.,Liu, Zixin.,Liu, Kuo.,He, Lingjuan.,...&Zhou, Bin.(2023).Genetic dissection of intercellular interactions in vivo by membrane-permeable protein.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA;,120(1).
MLA	Zhang, Shaohua,et al."Genetic dissection of intercellular interactions in vivo by membrane-permeable protein".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA; 120.1(2023).