Ring-stacked capsids of white spot syndrome virus and structural transitions with genome ejection

doi:10.1126/sciadv.add2796

	Ring-stacked capsids of white spot syndrome virus and structural transitions with genome ejection
	Sun, Meiling 1; Liu, Mingdong2,3,4 ; Shan, Hong4 ; Li, Kang 3,5; Wang, Peng 1; Guo, Huarong 6,7; Zhao, Yaqi 6; Wang, Rui4 ; Tao, Yiwen 6; Yang, Liuyan 5; Zhang, Ying4 ; Su, Xiaoming 8; Liu, Yunhui2,3 ; Li, Chunyang 1; Lin, James 8; Chen, Xiu-Lan 5; Zhang, Yu-Zhong 1,3,5; Shen, Qing-Tao2,3,4
	2023-02
发表期刊	SCIENCE ADVANCES (IF:11.7[JCR-2023],13.7[5-Year])
ISSN	2375-2548
EISSN	2375-2548
卷号	9 期号:8
发表状态	已发表
DOI	10.1126/sciadv.add2796
摘要	White spot syndrome virus (WSSV) is one of the largest DNA viruses and the major pathogen responsible for white spot syndrome in crustaceans. The WSSV capsid is critical for genome encapsulation and ejection and exhibits the rod-shaped and oval-shaped structures during the viral life cycle. However, the detailed architecture of the capsid and the structural transition mechanism remain unclear. Here, using cryo-electron microscopy (cryo-EM), we obtained a cryo-EM model of the rod-shaped WSSV capsid and were able to characterize its ring-stacked assembly mechanism. Furthermore, we identified an oval-shaped WSSV capsid from intact WSSV virions and analyzed the structural transition mechanism from the oval-shaped to rod-shaped capsids induced by high salinity. These transitions, which decrease internal capsid pressure, always accompany DNA release and mostly eliminate the infection of the host cells. Our results demonstrate an unusual assembly mechanism of the WSSV capsid and offer structural insights into the pressure-driven genome release. © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science.
关键词	Electron microscopes Electron microscopy Genes Medical imaging Viruses Assembly mechanism Cryo-electron microscopy DNA virus Rod-shaped Shaped structures Structural transitions Transition mechanism Virus capsid White spot syndrome virus White spots
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收录类别	SCI ; EI ; SCOPUS
语种	英语
资助项目	National Key Research and Development Program of China[
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000948176900013
出版者	American Association for the Advancement of Science
EI入藏号	20231013663372
EI主题词	Life cycle
EI分类号	461.1 Biomedical Engineering ; 461.2 Biological Materials and Tissue Engineering ; 461.9 Biology ; 746 Imaging Techniques
原始文献类型	Journal article (JA)
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/284242
专题	iHuman研究所_公共科研平台_生命科学电镜平台生命科学与技术学院_公共科研平台_分子影像平台 iHuman研究所_PI研究组_沈庆涛组生命科学与技术学院_硕士生生命科学与技术学院_博士生
通讯作者	Zhang, Ying; Zhang, Yu-Zhong; Shen, Qing-Tao
作者单位	1.Frontiers Science Center for Deep Ocean Multispheres and Earth System & College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. 2.School of Life Science, Department of Biology, Southern University of Science and Technology, Shenzhen 518055, China. 3.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China 4.iHuman Institute and School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China. 5.State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, China. 6.Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. 7.Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China. 8.High Performance Computing Center, Shanghai Jiao Tong University, Shanghai 200240, China.
通讯作者单位	iHuman研究所
推荐引用方式 GB/T 7714	Sun, Meiling,Liu, Mingdong,Shan, Hong,et al. Ring-stacked capsids of white spot syndrome virus and structural transitions with genome ejection[J]. SCIENCE ADVANCES,2023,9(8).
APA	Sun, Meiling.,Liu, Mingdong.,Shan, Hong.,Li, Kang.,Wang, Peng.,...&Shen, Qing-Tao.(2023).Ring-stacked capsids of white spot syndrome virus and structural transitions with genome ejection.SCIENCE ADVANCES,9(8).
MLA	Sun, Meiling,et al."Ring-stacked capsids of white spot syndrome virus and structural transitions with genome ejection".SCIENCE ADVANCES 9.8(2023).