CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging

doi:10.1038/s41592-018-0174-0

	CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging
	Ma, Hanhui1,2 ; Tu, Li-Chun 3; Naseri, Ardalan 4; Chung, Yu-Chieh 5; Grunwald, David 3; Zhang, Shaojie 4; Pederson, Thoru 1
	2018-11
发表期刊	NATURE METHODS (IF:36.1[JCR-2023],45.6[5-Year])
ISSN	1548-7091
卷号	15 期号:11 页码:928-+
发表状态	已发表
DOI	10.1038/s41592-018-0174-0
摘要	Clustered regularly interspaced short palindromic repeats (CRISPR) guide RNA scaffolds have been adapted to carry multiple binding sites for fluorescent proteins to enhance brightness for live cell imaging of genomic loci. However, many of these modifications result in guide RNA instability and thus produce lower genome-labeling efficiency than anticipated. Here we introduce CRISPR-Sirius, based on octet arrays of aptamers conferring both enhanced guide RNA stability and brightness, and provide initial biological applications of this platform.
收录类别	SCI ; SCIE
语种	英语
资助项目	National Institutes of Health (NIH)[U01 DA-040588] ; National Institutes of Health (NIH)[R01 GM102515] ; National Institutes of Health (NIH)[U01 EB021238] ; National Institutes of Health (NIH)[K99 GM126810]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
WOS记录号	WOS:000448827300029
出版者	NATURE PUBLISHING GROUP
WOS关键词	COAT PROTEIN ; LIVE-CELL ; SEQUENCES ; LOCI ; ORGANIZATION ; ARCHITECTURE ; RECOGNITION ; CHROMOSOMES ; EXPRESSION ; SELECTION
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/28294
专题	生命科学与技术学院_PI研究组_马涵慧组
通讯作者	Ma, Hanhui
作者单位	1.Univ Massachusetts, Sch Med, Dept Biochem & Mol Pharmacol, Worcester, MA 01655 USA 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 3.Univ Massachusetts, Med Sch, RNA Therapeut Inst, Worcester, MA USA 4.Univ Cent Florida, Dept Comp Sci, Orlando, FL 32816 USA 5.Univ Tokyo, Kavli Inst Phys & Math Universe, Kashiwa, Chiba, Japan
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Ma, Hanhui,Tu, Li-Chun,Naseri, Ardalan,et al. CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging[J]. NATURE METHODS,2018,15(11):928-+.
APA	Ma, Hanhui.,Tu, Li-Chun.,Naseri, Ardalan.,Chung, Yu-Chieh.,Grunwald, David.,...&Pederson, Thoru.(2018).CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.NATURE METHODS,15(11),928-+.
MLA	Ma, Hanhui,et al."CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging".NATURE METHODS 15.11(2018):928-+.