Transcription initiation defines kinetoplast RNA boundaries

doi:10.1073/pnas.1808981115

	Transcription initiation defines kinetoplast RNA boundaries
	Sement, Francois M.1; Suematsu, Takuma 1; Zhang, Liye2 ; Yu, Tian 1,3; Huang, Lan 4; Aphasizheva, Inna 1; Aphasizhev, Ruslan 1,5
	2018-10-30
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (IF:9.4[JCR-2023],10.8[5-Year])
ISSN	0027-8424
卷号	115 期号:44 页码:E10323-E10332
发表状态	已发表
DOI	10.1073/pnas.1808981115
摘要	Mitochondrial genomes are often transcribed into polycistronic RNAs punctuated by tRNAs whose excision defines mature RNA boundaries. Although kinetoplast DNA lacks tRNA genes, it is commonly held that in Trypanosoma brucei the monophosphorylated 5' ends of functional molecules typify precursor partitioning by an unknown endonuclease. On the contrary, we demonstrate that individual mRNA5 and rRNAs are independently synthesized as 3'-extended precursors. The transcription-defined 5' terminus is converted into a monophosphorylated state by the pyrophosphohydrolase complex, termed the "PPsome." Composed of the MERS1 NUDIX enzyme, the MERS2 pentatricopeptide repeat RNA-binding subunit, and MERS3 polypeptide, the PPsome binds to specific sequences near mRNA 5' termini. Most guide RNAs lack PPsome-recognition sites and remain triphosphorylated. The RNA-editing substrate-binding complex stimulates MERS1 pyrophosphohydrolase activity and enables an interaction between the PPsome and the polyadenylation machinery. We provide evidence that both 5' pyrophosphate removal and 3' adenylation are essential for mRNA stabilization. Furthermore, we uncover a mechanism by which antisense RNA-controlled 3'-5' exonucleolytic trimming defines the mRNA 3' end before adenylation. We conclude that mitochondria! mRNAs and rRNAs are transcribed and processed as insulated units irrespective of their genomic location.
关键词	Trypanosoma mitochondria transcription RNA stability NUDIX hydrolase
收录类别	SCI ; SCIE
语种	英语
资助项目	NIH[R01 AI113157] ; NIH[R01 GM074830] ; NIH[R01 GM106003] ; NIH[R01 AI091914] ; NIH[R01 AI101057]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000448713200005
出版者	NATL ACAD SCIENCES
WOS关键词	TRYPANOSOMA-BRUCEI ; LEISHMANIA-TARENTOLAE ; MAXICIRCLE DNA ; POLYADENYLATION ; MITOCHONDRIA ; PROTEINS ; COMPLEX ; BINDING ; TRANSLATION ; EXPRESSION
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/28291
专题	生命科学与技术学院_PI研究组_张力烨组
通讯作者	Aphasizhev, Ruslan
作者单位	1.Boston Univ, Dept Mol & Cell Biol, Boston, MA 02118 USA 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China 3.Boston Univ, Bioinformat Program, Boston, MA 02215 USA 4.Univ Calif Irvine, Sch Med, Dept Physiol & Biophys, Irvine, CA 92697 USA 5.Boston Univ, Dept Biochem, Boston, MA 02118 USA
推荐引用方式 GB/T 7714	Sement, Francois M.,Suematsu, Takuma,Zhang, Liye,et al. Transcription initiation defines kinetoplast RNA boundaries[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2018,115(44):E10323-E10332.
APA	Sement, Francois M..,Suematsu, Takuma.,Zhang, Liye.,Yu, Tian.,Huang, Lan.,...&Aphasizhev, Ruslan.(2018).Transcription initiation defines kinetoplast RNA boundaries.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,115(44),E10323-E10332.
MLA	Sement, Francois M.,et al."Transcription initiation defines kinetoplast RNA boundaries".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115.44(2018):E10323-E10332.