A dual genetic tracing system identifies diverse and dynamic origins of cardiac valve mesenchyme
2018-09
发表期刊DEVELOPMENT (IF:3.7[JCR-2023],5.0[5-Year])
ISSN0950-1991
卷号145期号:18
发表状态已发表
DOI10.1242/dev.167775
摘要In vivo genomic engineering is instrumental for studying developmental biology and regenerative medicine. Development of novel systems with more site-specific recombinases (SSRs) that complement with the commonly used Cre-loxP would be valuable for more precise lineage tracing and genome editing. Here, we introduce a new SSR system via Nigri-nox. By generating tissue-specific Nigri knock-in and its responding nox reporter mice, we show that the Nigri-nox system works efficiently in vivo by targeting specific tissues. As a new orthogonal system to Cre-loxP, Nigri-nox provides an additional control of genetic manipulation. We also demonstrate how the two orthogonal systems Nigri-nox and Cre-loxP could be used simultaneously to map the cell fate of two distinct developmental origins of cardiac valve mesenchyme in the mouse heart, providing dynamics of cellular contribution from different origins for cardiac valve mesenchyme during development. This work provides a proofof-principle application of the Nigri-nox system for in vivo mouse genomic engineering. Coupled with other SSR systems, Nigri-nox would be valuable for more precise delineation of origins and cell fates during development, diseases and regeneration.
关键词Genetic engineering Site-specific recombinase (SSR) Nigri-nox Fate mapping Cardiac valve development Genetic engineering Site-specific recombinase (SSR) Nigri-nox Fate mapping Cardiac valve development
收录类别SCI ; SCIE
语种英语
资助项目Health and Medical Research Fund[03140346] ; Health and Medical Research Fund[04152566]
WOS研究方向Developmental Biology
WOS类目Developmental Biology
WOS记录号WOS:000447270800014
出版者COMPANY BIOLOGISTS LTD
WOS关键词SITE-SPECIFIC RECOMBINASE ; NEURAL CREST ; EPICARDIAL PROGENITORS ; MAMMALIAN-CELLS ; STEM-CELLS ; LINEAGE ; HEART ; MICE ; EXPRESSION ; FATE
原始文献类型Article
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文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/28111
专题生命科学与技术学院_特聘教授组_周斌组
通讯作者Tian, Xueying; Zhou, Bin
作者单位
1.Univ Chinese Acad Sci, Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, State Key Lab Cell Biol,Shanghai Inst Biochem & C, Shanghai 200031, Peoples R China
2.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Nutr Sci, Key Lab Nutr & Metab,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China
3.East China Normal Univ, Sch Life Sci, Shanghai 200241, Peoples R China
4.Jinan Univ, Key Lab Regenerat Med, Minist Educ, Guangzhou 510632, Guangdong, Peoples R China
5.Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA
6.Chinese Univ Hong Kong, Prince Wales Hosp, Dept Chem Pathol, Li Ka Shing Inst Hlth Sci, Shatin, Hong Kong, Peoples R China
7.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
通讯作者单位生命科学与技术学院
推荐引用方式
GB/T 7714
Liu, Kuo,Yu, Wei,Tang, Muxue,et al. A dual genetic tracing system identifies diverse and dynamic origins of cardiac valve mesenchyme[J]. DEVELOPMENT,2018,145(18).
APA Liu, Kuo.,Yu, Wei.,Tang, Muxue.,Tang, Juan.,Liu, Xiuxiu.,...&Zhou, Bin.(2018).A dual genetic tracing system identifies diverse and dynamic origins of cardiac valve mesenchyme.DEVELOPMENT,145(18).
MLA Liu, Kuo,et al."A dual genetic tracing system identifies diverse and dynamic origins of cardiac valve mesenchyme".DEVELOPMENT 145.18(2018).
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