A post-ingestive amino acid sensor promotes food consumption in Drosophila

doi:10.1038/s41422-018-0084-9

	A post-ingestive amino acid sensor promotes food consumption in Drosophila
	Yang, Zhe 1,2; Huang, Rui 3,4; Fu, Xin5,6,7 ; Wang, Gaohang 1,2; Qi, Wei 1,2; Mao, Decai 8; Shi, Zhaomei5 ; Shen, Wei L.5 ; Wang, Liming 1,2
	2018-10
发表期刊	CELL RESEARCH (IF:28.1[JCR-2023],36.4[5-Year])
ISSN	1001-0602
卷号	28 期号:10 页码:1013-1025
发表状态	已发表
DOI	10.1038/s41422-018-0084-9
摘要	Adequate protein intake is crucial for the survival and well-being of animals. How animals assess prospective protein sources and ensure dietary amino acid intake plays a critical role in protein homeostasis. By using a quantitative feeding assay, we show that three amino acids, L-glutamate (L-Glu), L-alanine (L-Ala) and L-aspartate (L-Asp), but not their D-enantiomers or the other 17 natural L-amino acids combined, rapidly promote food consumption in the fruit fly Drosophila melanogaster. This feeding-promoting effect of dietary amino acids is independent of mating experience and internal nutritional status. In vivo and ex vivo calcium imagings show that six brain neurons expressing diuretic hormone 44 (DH44) can be rapidly and directly activated by these amino acids, suggesting that these neurons are an amino acid sensor. Genetic inactivation of DH44(+) neurons abolishes the increase in food consumption induced by dietary amino acids, whereas genetic activation of these neurons is sufficient to promote feeding, suggesting that DH44(+) neurons mediate the effect of dietary amino acids to promote food consumption. Single-cell transcriptome analysis and immunostaining reveal that a putative amino acid transporter, CG13248, is enriched in DH44(+) neurons. Knocking down CG13248 expression in DH44( )(+)neurons blocks the increase in food consumption and eliminates calcium responses induced by dietary amino acids. Therefore, these data identify DH44(+) neuron as a key sensor to detect amino acids and to enhance food intake via a putative transporter CG13248. These results shed critical light on the regulation of protein homeostasis at organismal levels by the nervous system.
收录类别	SCI ; SCIE ; CSCD
语种	英语
资助项目	Fundamental Research Funds for the Central Universities[2016QN81010] ; Fundamental Research Funds for the Central Universities[2015XZZX004-33]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000446172500007
CSCD记录号	CSCD:6366207
出版者	INST BIOCHEMISTRY & CELL BIOLOGY
WOS关键词	ADULT DROSOPHILA ; LARVAL DROSOPHILA ; CIRCADIAN CLOCK ; S6 KINASE ; TASTE ; NEURONS ; DEFICIENCY ; BEHAVIOR ; HUNGER ; CELLS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/28070
专题	生命科学与技术学院_博士生生命科学与技术学院_PI研究组_沈伟组生命科学与技术学院_公共科研平台_组学分析平台
通讯作者	Shen, Wei L.; Wang, Liming
作者单位	1.Zhejiang Univ, Life Sci Inst, Hangzhou 310058, Zhejiang, Peoples R China 2.Zhejiang Univ, Innovat Ctr Cell Signaling Network, Hangzhou 310058, Zhejiang, Peoples R China 3.Chongqing Univ, Bioengn Coll, State & Local Joint Engn Lab Vasc Implants, Key Lab Biorheol Sci & Technol,Minist Educ, Chongqing 400030, Peoples R China 4.Chongqing Univ, Med Sch, Chongqing 400030, Peoples R China 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 6.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Shanghai 200031, Peoples R China 7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 8.Tsinghua Univ, Sch Med, Gene Regulatory Lab, Beijing 100084, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Yang, Zhe,Huang, Rui,Fu, Xin,et al. A post-ingestive amino acid sensor promotes food consumption in Drosophila[J]. CELL RESEARCH,2018,28(10):1013-1025.
APA	Yang, Zhe.,Huang, Rui.,Fu, Xin.,Wang, Gaohang.,Qi, Wei.,...&Wang, Liming.(2018).A post-ingestive amino acid sensor promotes food consumption in Drosophila.CELL RESEARCH,28(10),1013-1025.
MLA	Yang, Zhe,et al."A post-ingestive amino acid sensor promotes food consumption in Drosophila".CELL RESEARCH 28.10(2018):1013-1025.