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ShanghaiTech University Knowledge Management System
| Mapping the Complete Photocycle that Powers a Large Stokes Shift Red Fluorescent Protein | |
| 2022-12-01 | |
| 发表期刊 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (IF:16.1[JCR-2023],16.2[5-Year]) |
| ISSN | 1433-7851 |
| EISSN | 1521-3773 |
| 卷号 | 62期号:5 |
| 发表状态 | 已发表 |
| DOI | 10.1002/anie.202212209 |
| 摘要 | Large Stokes shift (LSS) red fluorescent proteins (RFPs) are highly desirable for bioimaging advances. The RFP mKeima, with coexisting cis- and trans-isomers, holds significance as an archetypal system for LSS emission due to excited-state proton transfer (ESPT), yet the mechanisms remain elusive. We implemented femtosecond stimulated Raman spectroscopy (FSRS) and various time-resolved electronic spectroscopies, aided by quantum calculations, to dissect the cis- and trans-mKeima photocycle from ESPT, isomerization, to ground-state proton transfer in solution. This work manifests the power of FSRS with global analysis to resolve Raman fingerprints of intermediate states. Importantly, the deprotonated trans-isomer governs LSS emission at 620 nm, while the deprotonated cis-isomer's 520 nm emission is weak due to an ultrafast cis-to-trans isomerization. Complementary spectroscopic techniques as a table-top toolset are thus essential to study photochemistry in physiological environments. |
| 关键词 | Chromophore Isomerization Excited- and Ground-State Proton Transfer Femtosecond Raman Spectroscopy Large Stokes Shift Red Fluorescent Proteins |
| URL | 查看原文 |
| 收录类别 | SCI ; EI ; SCOPUS |
| 语种 | 英语 |
| 资助项目 | Natural Science Foundation of Shanghai[20ZR1436300] ; U.S. National Science Foundation[MCB-1817949] ; National Natural Science Foundation of China[ |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Multidisciplinary |
| WOS记录号 | WOS:000902343000001 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| EI入藏号 | 20225213306109 |
| EI主题词 | Excited states |
| EI分类号 | 741.1 Light/Optics ; 802.2 Chemical Reactions ; 804 Chemical Products Generally ; 804.1 Organic Compounds ; 931.3 Atomic and Molecular Physics ; 931.4 Quantum Theory ; Quantum Mechanics ; 932.1 High Energy Physics |
| 原始文献类型 | Journal article (JA) |
| 引用统计 | 正在获取...
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| 文献类型 | 期刊论文 |
| 条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/278841 |
| 专题 | 物质科学与技术学院_PI研究组_刘伟民组 物质科学与技术学院_PI研究组_季泉江组 物质科学与技术学院_PI研究组_黄逸凡组 物质科学与技术学院_博士生 大科学中心_PI研究组_翁祖谦组 大科学中心_公共科研平台_大科学装置建设部 |
| 通讯作者 | Ji, Quanjiang; Huang, Yifan; Fang, Chong; Liu, Weimin |
| 作者单位 | 1.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Oregon State Univ, Dept Chem, 153 Gilbert Hall, Corvallis, OR 97331 USA |
| 第一作者单位 | 物质科学与技术学院 |
| 通讯作者单位 | 物质科学与技术学院 |
| 第一作者的第一单位 | 物质科学与技术学院 |
| 推荐引用方式 GB/T 7714 | Wang, Ziyu,Zhang, Ya,Chen, Cheng,et al. Mapping the Complete Photocycle that Powers a Large Stokes Shift Red Fluorescent Protein[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2022,62(5). |
| APA | Wang, Ziyu.,Zhang, Ya.,Chen, Cheng.,Zhu, Ruixue.,Jiang, Jiaming.,...&Liu, Weimin.(2022).Mapping the Complete Photocycle that Powers a Large Stokes Shift Red Fluorescent Protein.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,62(5). |
| MLA | Wang, Ziyu,et al."Mapping the Complete Photocycle that Powers a Large Stokes Shift Red Fluorescent Protein".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 62.5(2022). |
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