Genome-wide screening of NEAT1 regulators reveals cross-regulation between paraspeckles and mitochondria

doi:10.1038/s41556-018-0204-2

	Genome-wide screening of NEAT1 regulators reveals cross-regulation between paraspeckles and mitochondria
	Wang, Yang 1; Hu, Shi-Bin 1,4; Wang, Meng-Ran 2; Yao, Run-Wen 1; Wu, Di 1; Yang, Li2,3 ; Chen, Ling-Ling1,3
	2018-10
发表期刊	NATURE CELL BIOLOGY (IF:17.3[JCR-2023],24.2[5-Year])
ISSN	1465-7392
卷号	20 期号:10 页码:1145-+
发表状态	已发表
DOI	10.1038/s41556-018-0204-2
摘要	The long noncoding RNA NEAT1 (nuclear enriched abundant transcript 1) nucleates the formation of paraspeckles, which constitute a type of nuclear body with multiple roles in gene expression. Here we identify NEAT1 regulators using an endogenous NEAT1 promoter-driven enhanced green fluorescent protein reporter in human cells coupled with genome-wide RNAi screens. The screens unexpectedly yield gene candidates involved in mitochondrial functions as essential regulators of NEAT1 expression and paraspeckle formation. Depletion of mitochondrial proteins and treatment of mitochondrial stressors both lead to aberrant NEAT1 expression via ATF2 as well as altered morphology and numbers of paraspeckles. These changes result in enhanced retention of mRNAs of nuclear-encoded mitochondrial proteins (mito-mRNAs) in paraspeckles. Correspondingly, NEAT1 depletion has profound effects on mitochondrial dynamics and function by altering the sequestration of mito-mRNAs in paraspeckles. Overall, our data provide a rich resource for understanding NEAT1 and paraspeckle regulation, and reveal a cross-regulation between paraspeckles and mitochondria.
收录类别	SCI ; SCIE
语种	英语
资助项目	Howard Hughes Medical Institute[55008728]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000445656400010
出版者	NATURE PUBLISHING GROUP
WOS关键词	LONG NONCODING RNA ; NUCLEAR RETENTION ; MESSENGER-RNAS ; CELL-DEATH ; PROTEIN ; EXPRESSION ; SUBPOPULATION ; TRANSCRIPTION ; LOCALIZATION ; DISTINCT
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/27787
专题	生命科学与技术学院_特聘教授组_杨力组生命科学与技术学院_特聘教授组_陈玲玲组
通讯作者	Chen, Ling-Ling
作者单位	1.Chinese Acad Sci, State Key Lab Mol Biol, Shanghai Key Lab Mol Androl,Univ Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci,Shanghai Inst Bio, Shanghai, Peoples R China 2.Chinese Acad Sci, Key Lab Computat Biol, CAS MPG Partner Inst Computat Biol,Univ Chinese A, Shanghai Inst Nutr & Hlth,Shanghai Inst Biol Sci, Shanghai, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 4.Stanford Univ, Dept Genet, Stanford, CA 94305 USA
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wang, Yang,Hu, Shi-Bin,Wang, Meng-Ran,et al. Genome-wide screening of NEAT1 regulators reveals cross-regulation between paraspeckles and mitochondria[J]. NATURE CELL BIOLOGY,2018,20(10):1145-+.
APA	Wang, Yang.,Hu, Shi-Bin.,Wang, Meng-Ran.,Yao, Run-Wen.,Wu, Di.,...&Chen, Ling-Ling.(2018).Genome-wide screening of NEAT1 regulators reveals cross-regulation between paraspeckles and mitochondria.NATURE CELL BIOLOGY,20(10),1145-+.
MLA	Wang, Yang,et al."Genome-wide screening of NEAT1 regulators reveals cross-regulation between paraspeckles and mitochondria".NATURE CELL BIOLOGY 20.10(2018):1145-+.