A proximity-tagging system to identify membrane protein-protein interactions

doi:10.1038/s41592-018-0100-5

	A proximity-tagging system to identify membrane protein-protein interactions
	Liu, Qiang1,2,3 ; Zheng, Jun1,3 ; Sun, Weiping1,3 ; Huo, Yinbo1,2,3 ; Zhang, Liye1 ; Hao, Piliang1 ; Wang, Haopeng1 ; Zhuang, Min1
	2018-09
发表期刊	NATURE METHODS
ISSN	1548-7091
卷号	15 期号:9 页码:715-+
发表状态	已发表
DOI	10.1038/s41592-018-0100-5
摘要	The communication between cells and between cellular organelles is often controlled by the interaction of membrane proteins. Although many methods for the detection of protein-protein interactions (PPIs) exist, membrane PPIs remain difficult to detect. Here we developed a proximity-based tagging system, PUP-IT (pupylation-based interaction tagging), to identify membrane protein interactions. In this approach, a small protein tag, Pup, is applied to proteins that interact with a PafA-fused bait, enabling transient and weak interactions to be enriched and detected by mass spectrometry. Pup does not diffuse from the enzyme, which allows high-specificity labeling. We applied this approach to CD28, a critical costimulatory receptor for T lymphocyte activation, and identified known CD28 binding partners and multiple potential interacting proteins. In addition, we demonstrated that this method can identify the interaction between a cell surface receptor and its ligand.
收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[31570767] ; National Natural Science Foundation of China[31670919]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
WOS记录号	WOS:000443439700029
出版者	NATURE PUBLISHING GROUP
WOS关键词	UBIQUITIN-LIKE PROTEIN ; MYCOBACTERIUM-TUBERCULOSIS ; PROTEOMIC ANALYSIS ; MAMMALIAN-CELLS ; BIOTIN LIGASE ; LIVING CELLS ; E3 LIGASE ; IDENTIFICATION ; DEGRADATION ; COMPLEX
原始文献类型	Article
引用统计	被引频次：129[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/27694
专题	生命科学与技术学院生命科学与技术学院_PI研究组_张力烨组生命科学与技术学院_PI研究组_庄敏组生命科学与技术学院_PI研究组_王皞鹏组生命科学与技术学院_公共科研平台_组学分析平台生命科学与技术学院_硕士生生命科学与技术学院_博士生
通讯作者	Wang, Haopeng; Zhuang, Min
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Liu, Qiang,Zheng, Jun,Sun, Weiping,et al. A proximity-tagging system to identify membrane protein-protein interactions[J]. NATURE METHODS,2018,15(9):715-+.
APA	Liu, Qiang.,Zheng, Jun.,Sun, Weiping.,Huo, Yinbo.,Zhang, Liye.,...&Zhuang, Min.(2018).A proximity-tagging system to identify membrane protein-protein interactions.NATURE METHODS,15(9),715-+.
MLA	Liu, Qiang,et al."A proximity-tagging system to identify membrane protein-protein interactions".NATURE METHODS 15.9(2018):715-+.