Necessary Experimental Conditions for Single-Shot Diffraction Imaging of DNA-Based Structures with X-ray Free-Electron Lasers

doi:10.1021/acsnano.8b01838

	Necessary Experimental Conditions for Single-Shot Diffraction Imaging of DNA-Based Structures with X-ray Free-Electron Lasers
	Sun, Zhibin1,2,3 ; Fan, Jiadong3 ; Li, Haoyuan 2,4; Liu, Huajie 5; Nam, Daewoong 6,7; Kim, Chan 8,9,10; Kim, Yoonhee 8,9,10; Han, Yubo 11,12; Zhang, Jianhua1,3 ; Yao, Shengkun3 ; Park, Jaehyun 6; Kim, Sunam 6; Tono, Kensuke 13; Yabashi, Makina 14; Ishikawa, Tetsuya 14; Song, Changyong 7; Fan, Chunhai 5; Jiang, Huaidong3
	2018-08
发表期刊	ACS NANO (IF:15.8[JCR-2023],16.2[5-Year])
ISSN	1936-0851
卷号	12 期号:8 页码:7509-7518
发表状态	已发表
DOI	10.1021/acsnano.8b01838
摘要	It has been proposed that the radiation damage to biological particles and soft condensed matter can be overcome by ultrafast and ultraintense X-ray free-electron lasers (FELs) with short pulse durations. The successful demonstration of the "diffraction-before-destruction" concept has made single-shot diffraction imaging a promising tool to achieve high resolutions under the native states of samples. However, the resolution is still limited because of the low signal-to-noise ratio, especially for biological specimens such as cells, viruses, and macromolecular partides. Here, we present a demonstration single-shot diffraction imaging experiment of DNA-based structures at SPring-8 Angstrom Compact Free Electron Laser (SACLA), Japan. Through quantitative analysis of the reconstructed images, the scattering abilities of gold and DNA were demonstrated. Suggestions for extracting valid DNA signals from noisy diffraction patterns were also explained and outlined. To sketch out the necessary experimental conditions for the 3D imaging of DNA origami or DNA macromolecular particles, we carried out numerical simulations with practical detector noise and experimental geometry using the Linac Coherent Light Source (LCLS) at the SLAC National Accelerator Laboratory, USA. The simulated results demonstrate that it is possible to capture images of DNA-based structures at high resolutions with the technique development of current and next-generation X-ray FEL facilities.
关键词	DNA origami X-ray free-electron laser single particle imaging coherent diffraction imaging X-ray diffraction
收录类别	SCI ; SCIE ; EI
语种	英语
资助项目	U.S. Department of Energy, Office of Basic Energy Sciences[DE-AC02-76SF00515]
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science
WOS类目	Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS记录号	WOS:000443525600007
出版者	AMER CHEMICAL SOC
EI入藏号	20182905561782
EI主题词	Condensed matter physics ; Diffraction ; DNA ; Electrons ; Free electron lasers ; Radiation damage ; Signal processing ; Signal to noise ratio ; Viruses ; X rays
WOS关键词	COHERENT-LIGHT SOURCE ; PLASMONIC NANOSTRUCTURES ; ORIGAMI ; CRYSTALLOGRAPHY ; PULSES ; RECONSTRUCTION ; NANOPARTICLES ; ORIENTATION ; INSTRUMENT ; RESOLUTION
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/27690
专题	大科学中心_PI研究组_江怀东组物质科学与技术学院硬x射线自由电子激光装置项目
通讯作者	Jiang, Huaidong
作者单位	1.Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Shandong, Peoples R China 2.SLAC Natl Accelerator Lab, Linac Coherent Light Source, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA 3.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China 4.Stanford Univ, Dept Phys, Stanford, CA 94305 USA 5.Chinese Acad Sci, Shanghai Inst Appl Phys, Phys Biol Lab, Shanghai 201800, Peoples R China 6.Pohang Univ Sci & Technol, Pohang Accelerator Lab, Pohang 37673, South Korea 7.Pohang Univ Sci & Technol, Dept Phys, Pohang 37673, South Korea 8.European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld, Germany 9.Gwangju Inst Sci & Technol, Dept Phys & Photon Sci, Gwangju 61005, South Korea 10.Gwangju Inst Sci & Technol, Sch Mat Sci & Engn, Gwangju 61005, South Korea 11.Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China 12.SLAC Natl Accelerator Lab, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA 13.Japan Synchrotron Radiat Res Inst, Sayo, Hyogo 6795198, Japan 14.RIKEN SPring 8 Ctr, Sayo, Hyogo 6795148, Japan
第一作者单位	物质科学与技术学院
通讯作者单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Sun, Zhibin,Fan, Jiadong,Li, Haoyuan,et al. Necessary Experimental Conditions for Single-Shot Diffraction Imaging of DNA-Based Structures with X-ray Free-Electron Lasers[J]. ACS NANO,2018,12(8):7509-7518.
APA	Sun, Zhibin.,Fan, Jiadong.,Li, Haoyuan.,Liu, Huajie.,Nam, Daewoong.,...&Jiang, Huaidong.(2018).Necessary Experimental Conditions for Single-Shot Diffraction Imaging of DNA-Based Structures with X-ray Free-Electron Lasers.ACS NANO,12(8),7509-7518.
MLA	Sun, Zhibin,et al."Necessary Experimental Conditions for Single-Shot Diffraction Imaging of DNA-Based Structures with X-ray Free-Electron Lasers".ACS NANO 12.8(2018):7509-7518.