Exploration of phenol tolerance mechanism through antioxidative responses of an evolved strain, Chlorella sp L5

doi:10.1007/s10811-018-1428-z

	Exploration of phenol tolerance mechanism through antioxidative responses of an evolved strain, Chlorella sp L5
	Zhou, Lin 1,2; Yuan, Yizhong1,3,4 ; Li, Xuyang 1,2; Mei, Shouhua 1; Gao, Juan 2; Zhao, Quanyu1,4 ; Wei, Wei1,4,5 ; Sun, Yuhan1,4
	2018-08
发表期刊	JOURNAL OF APPLIED PHYCOLOGY (IF:2.8[JCR-2023],3.2[5-Year])
ISSN	0921-8971
卷号	30 期号:4 页码:2379-2385
发表状态	已发表
DOI	10.1007/s10811-018-1428-z
摘要	Phenol is a typical pollutant which can be degraded by microalgae. Phenol induces formation of reactive oxygen species (ROS). Tolerance to oxidative damage produced by ROS is critical for phenol degradation by microalgae. An evolved strain, Chlorella sp. L5 was obtained from Chlorella sp. L3 in a previous study. The current study investigated the phenol tolerance mechanism by the dynamic responses of antioxidative enzyme activities of Chlorella sp. L3 and Chlorella sp. L5 in TAP medium with and without 500 mg L-1 phenol. It was shown that the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were increased in Chlorella sp. L5 when phenol was added in the medium. They were also significantly higher than those of Chlorella sp. L3 under 500 mg L-1 phenol conditions. There were slight increases of the malondialdehyde (MDA) contents of Chlorella sp. L5 in TAP medium with and without 500 mg L-1 phenol while they were significantly lower than the MDA content of Chlorella sp. L3 under 500 mg L-1 phenol conditions. The results indicated that Chlorella sp. L5 had higher tolerance to 500 mg L-1 phenol than Chlorella sp. L3. The antioxidative responses of Chlorella sp. L3 and Chlorella sp. L5 in control and TAP medium + phenol were consisted with the results of the comparative transcriptomic analysis in another published result. The phenol tolerance mechanism was also confirmed by the photosynthetic efficiency under oxidative conditions and control.
关键词	Chlorella Phenol tolerance Antioxidant Adaptive evolution
收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[21576278]
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:000440782100021
出版者	SPRINGER
WOS关键词	ADAPTIVE LABORATORY EVOLUTION ; INDUCED OXIDATIVE STRESS ; DUNALIELLA-SALINA ; CHLOROPHYLL FLUORESCENCE ; SALT STRESS ; DEGRADATION ; ENZYMES ; GROWTH ; ACCUMULATION ; PYRENOIDOSA
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/27616
专题	生命科学与技术学院物质科学与技术学院_特聘教授组_孙予罕组物质科学与技术学院_特聘教授组_魏伟组生命科学与技术学院_特聘教授组_赵权宇组生命科学与技术学院_硕士生
通讯作者	Zhao, Quanyu
作者单位	1.Chinese Acad Sci, Shanghai Adv Res Inst, 99 Haike Rd, Shanghai 201210, Peoples R China 2.Shanghai Univ, Sch Life Sci, 99 Shangda Rd, Shanghai 200444, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China 4.ShanghaiTech Univ, 100 Haike Rd, Shanghai 201210, Peoples R China 5.Chinese Acad Sci, Ctr Excellence Urban Atmospher Environm, Inst Urban Environm, Xiamen 361021, Peoples R China
通讯作者单位	上海科技大学
推荐引用方式 GB/T 7714	Zhou, Lin,Yuan, Yizhong,Li, Xuyang,et al. Exploration of phenol tolerance mechanism through antioxidative responses of an evolved strain, Chlorella sp L5[J]. JOURNAL OF APPLIED PHYCOLOGY,2018,30(4):2379-2385.
APA	Zhou, Lin.,Yuan, Yizhong.,Li, Xuyang.,Mei, Shouhua.,Gao, Juan.,...&Sun, Yuhan.(2018).Exploration of phenol tolerance mechanism through antioxidative responses of an evolved strain, Chlorella sp L5.JOURNAL OF APPLIED PHYCOLOGY,30(4),2379-2385.
MLA	Zhou, Lin,et al."Exploration of phenol tolerance mechanism through antioxidative responses of an evolved strain, Chlorella sp L5".JOURNAL OF APPLIED PHYCOLOGY 30.4(2018):2379-2385.