BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity

doi:10.1038/s41422-018-0052-4

	BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity
	Jiang, Wen 1; Feng, Songjie2,3,4 ; Huang, Shisheng2 ; Yu, Wenxia2 ; Li, Guanglei2 ; Yang, Guang2 ; Liu, Yajing2,3,4 ; Zhang, Yu2 ; Zhang, Lei 3,4; Hou, Yu 1; Chen, Jia2 ; Chen, Jieping 1; Huang, Xingxu2
	2018-08
发表期刊	CELL RESEARCH (IF:28.1[JCR-2023],36.4[5-Year])
ISSN	1001-0602
卷号	28 期号:8 页码:855-861
发表状态	已发表
DOI	10.1038/s41422-018-0052-4
摘要	Base editor (BE), containing a cytidine deaminase and catalytically defective Cas9, has been widely used to perform base editing. However, the narrow editing window of BE limits its utility. Here, we developed a new editing technology named as base editor for programming larger C to U (T) scope (BE-PLUS) by fusing 10 copies of GCN4 peptide to nCas9(D10A) for recruiting scFv-APOBEC-UGI-GB1 to the target sites. The new system achieves base editing with a broadened window, resulting in an increased genome-targeting scope. Interestingly, the new system yielded much fewer unwanted indels and non-C-to-T conversions. We also demonstrated its potential use in gene disruption across the whole genome through induction of stop codons (iSTOP). Taken together, the BE-PLUS system offers a new editing tool with increased editing window and enhanced fidelity.
收录类别	SCI ; SCIE ; CSCD
语种	英语
资助项目	Major technological innovation plan of hospital[SWH2016ZDCX1003] ; Major technological innovation plan of hospital[SWH2016ZDCX1010]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000441069300007
CSCD记录号	CSCD:6312713
出版者	INST BIOCHEMISTRY & CELL BIOLOGY
WOS关键词	MAMMALIAN-CELLS ; DEAMINASE FUSIONS ; HUMAN EMBRYOS ; TARGET BASE ; GENOME ; DNA ; PROTEIN ; SYSTEM ; HYPERMUTATION ; MUTATIONS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/27584
专题	生命科学与技术学院生命科学与技术学院_PI研究组_陈佳组生命科学与技术学院_PI研究组_黄行许组生命科学与技术学院_硕士生生命科学与技术学院_博士生
通讯作者	Chen, Jieping; Huang, Xingxu
作者单位	1.Army Med Univ, Mil Med Univ 3, Southwest Hosp, Dept Hematol, Chongqing 400038, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Jiang, Wen,Feng, Songjie,Huang, Shisheng,et al. BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity[J]. CELL RESEARCH,2018,28(8):855-861.
APA	Jiang, Wen.,Feng, Songjie.,Huang, Shisheng.,Yu, Wenxia.,Li, Guanglei.,...&Huang, Xingxu.(2018).BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity.CELL RESEARCH,28(8),855-861.
MLA	Jiang, Wen,et al."BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity".CELL RESEARCH 28.8(2018):855-861.