GPCR structural characterization by NMR spectroscopy in solution

doi:10.3724/abbs.2022106

	GPCR structural characterization by NMR spectroscopy in solution
	Yang, Lingyun1 ; Liu, Dongsheng1 ; Wuethrich, Kurt1,2,3
	2022-09-01
发表期刊	ACTA BIOCHIMICA ET BIOPHYSICA SINICA (IF:3.3[JCR-2023],3.4[5-Year])
ISSN	1672-9145
EISSN	1745-7270
卷号	54 期号:9
发表状态	已发表
DOI	10.3724/abbs.2022106
摘要	In the human proteome, 826 G-protein-coupled receptors (GPCRs) interact with extracellular stimuli to initiate cascades of intracellular signaling. Determining conformational dynamics and intermolecular interactions are key to understand GPCR function as a basis for drug design. X-ray crystallography and cryo-electron microscopy (cryoEM) contribute molecular architectures of GPCRs and GPCR-signaling complexes. NMR spectroscopy is complementary by providing information on the dynamics of GPCR structures at physiological temperature. In this review, several NMR approaches in use to probe GPCR dynamics and intermolecular interactions are discussed. The topics include uniform stable-isotope labeling, amino acid residue-selective stable-isotope labeling, site-specific labeling by genetic engineering, the introduction of F-19-NMR probes, and the use of paramagnetic nitroxide spin labels. The unique information provided by NMR spectroscopy contributes to our understanding of GPCR biology and thus adds to the foundations for rational drug design.
关键词	G protein-coupled receptor dynamics stable-isotope labeling fluorine-19 NMR GPCR biology drug development
URL	查看原文
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China["21904088","31971153"]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000892262800001
出版者	SCIENCE PRESS
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/268660
专题	iHuman研究所_公共科研平台_生命科学NMR平台 iHuman研究所_特聘教授组_Kurt Wuthrich组
通讯作者	Liu, Dongsheng
作者单位	1.ShanghaiTech Univ, iHuman Inst, Shanghai 201210, Peoples R China 2.Scripps Res, Dept Integrat Struct & Computat Biol, La Jolla, CA 92037 USA 3.Swiss Fed Inst Technol, Inst Mol Biol & Biophys, Otto Stern Weg 5, CH-8093 Zurich, Switzerland
第一作者单位	iHuman研究所
通讯作者单位	iHuman研究所
第一作者的第一单位	iHuman研究所
推荐引用方式 GB/T 7714	Yang, Lingyun,Liu, Dongsheng,Wuethrich, Kurt. GPCR structural characterization by NMR spectroscopy in solution[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2022,54(9).
APA	Yang, Lingyun,Liu, Dongsheng,&Wuethrich, Kurt.(2022).GPCR structural characterization by NMR spectroscopy in solution.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,54(9).
MLA	Yang, Lingyun,et al."GPCR structural characterization by NMR spectroscopy in solution".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 54.9(2022).