Kae1 of Saccharomyces cerevisiae KEOPS complex possesses ADP/GDP nucleotidase activity

doi:10.1042/BCJ20220290

	Kae1 of Saccharomyces cerevisiae KEOPS complex possesses ADP/GDP nucleotidase activity
	Li, Qian-Xi1 ; Liu, Jia-Cheng 2; He, Ming-Hong 2; Zhou, Jin-Qiu1,2,3
	2022-12-01
发表期刊	BIOCHEMICAL JOURNAL (IF:4.4[JCR-2023],3.7[5-Year])
ISSN	0264-6021
EISSN	1470-8728
卷号	479 期号:23
发表状态	已发表
DOI	10.1042/BCJ20220290
摘要	The KEOPS complex is an evolutionarily conserved protein complex in all three domains of life (Bacteria, Archaea, and Eukarya). In budding yeast Saccharomyces cerevisiae, the KEOPS complex (ScKEOPS) consists of five subunits, which are Kae1, Bud32, Cgi121, Pcc1, and Gon7. The KEOPS complex is an ATPase and is required for tRNA N6-threo-nylcarbamoyladenosine modification, telomere length maintenance, and efficient DNA repair. Here, recombinant ScKEOPS full complex and Kae1-Pcc1-Gon7 and Bud32- Cgi121 subcomplexes were purified and their biochemical activities were examined. KEOPS was observed to have ATPase and GTPase activities, which are predominantly attributed to the Bud32 subunit, as catalytically dead Bud32, but not catalytically dead Kae1, largely eliminated the ATPase/GTPase activity of KEOPS. In addition, KEOPS could hydrolyze ADP to adenosine or GDP to guanosine, and produce PPi, indicating that KEOPS is an ADP/GDP nucleotidase. Further mutagenesis characterization of Bud32 and Kae1 subunits revealed that Kae1, but not Bud32, is responsible for the ADP/GDP nucleotidase activity. In addition, the Kae1V309D mutant exhibited decreased ADP/GDP nucleotidase activity in vitro and shortened telomeres in vivo, but showed only a limited defect in t6A modification, suggesting that the ADP/GDP nucleotidase activity of KEOPS contributes to telomere length regulation.
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收录类别	SCI
语种	英语
资助项目	[2019YFA0109902] ; [3215000072]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000894651700001
出版者	PORTLAND PRESS LTD
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/266596
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_周金秋组
通讯作者	Zhou, Jin-Qiu
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 2.Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci,State Key Lab Mol, Shanghai 200031, Peoples R China 3.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Life Sci, Hangzhou 310024, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Li, Qian-Xi,Liu, Jia-Cheng,He, Ming-Hong,et al. Kae1 of Saccharomyces cerevisiae KEOPS complex possesses ADP/GDP nucleotidase activity[J]. BIOCHEMICAL JOURNAL,2022,479(23).
APA	Li, Qian-Xi,Liu, Jia-Cheng,He, Ming-Hong,&Zhou, Jin-Qiu.(2022).Kae1 of Saccharomyces cerevisiae KEOPS complex possesses ADP/GDP nucleotidase activity.BIOCHEMICAL JOURNAL,479(23).
MLA	Li, Qian-Xi,et al."Kae1 of Saccharomyces cerevisiae KEOPS complex possesses ADP/GDP nucleotidase activity".BIOCHEMICAL JOURNAL 479.23(2022).