The Aurora kinase inhibitor CYC116 promotes the maturation of cardiomyocytes derived from human pluripotent stem cells

doi:10.14348/molcells.2022.0077

	The Aurora kinase inhibitor CYC116 promotes the maturation of cardiomyocytes derived from human pluripotent stem cells
	Ji, Sijia1,2,3 ; Tu, Wanzhi1,2,3 ; Huang, Chenwen 2; Chen, Ziyang 2,3; Ren, Xinyue 2,3; He, Bingqing1,2,3 ; Ding, Xiaoyan 4; Chen, Yuelei 4; Xie, Xin1,2,3,5
	2022-12-31
发表期刊	MOLECULES AND CELLS (IF:3.7[JCR-2023],4.1[5-Year])
ISSN	1016-8478
EISSN	0219-1032
卷号	45 期号:12 页码:869~975
发表状态	已发表
DOI	10.14348/molcells.2022.0077
摘要	Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have great potential in applications such as regenerative medicine, cardiac disease modeling, and in vitro drug evaluation. However, hPSC-CMs are immature, which limits their applications. During development, the maturation of CMs is accompanied by a decline in their proliferative capacity. This phenomenon suggests that regulating the cell cycle may facilitate the maturation of hPSC-CMs. Aurora kinases are essential kinases that regulate the cell cycle, the role of which is not well studied in hPSC-CM maturation. Here, we demonstrate that CYC116, an inhibitor of Aurora kinases, significantly promotes the maturation of CMs derived from both human embryonic stem cells (H1 and H9) and iPSCs (induced PSCs) (UC013), resulting in increased expression of genes related to cardiomyocyte function, better organization of the sarcomere, increased sarcomere length, increased number of mitochondria, and enhanced physiological function of the cells. In addition, a number of other Aurora kinase inhibitors have also been found to promote the maturation of hPSC-CMs. Our data suggest that blocking aurora kinase activity and regulating cell cycle progression may promote the maturation of hPSC-CMs.
关键词	Aurora kinase inhibitor cardiomyocyte differen-tiation cell cycle human pluripotent stem cell maturation
URL	查看原文
收录类别	SCI
语种	英语
资助项目	[XDA16010202] ; [2017YFA0104002] ; [82121005] ; [81730099]
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000906898100001
出版者	KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/263285
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_谢欣组
通讯作者	Xie, Xin
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Biochem & Cell Biol, Stem Cell Bank Stem Cell Core Facil, Shanghai 200031, Peoples R China 5.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Ji, Sijia,Tu, Wanzhi,Huang, Chenwen,et al. The Aurora kinase inhibitor CYC116 promotes the maturation of cardiomyocytes derived from human pluripotent stem cells[J]. MOLECULES AND CELLS,2022,45(12):869~975.
APA	Ji, Sijia.,Tu, Wanzhi.,Huang, Chenwen.,Chen, Ziyang.,Ren, Xinyue.,...&Xie, Xin.(2022).The Aurora kinase inhibitor CYC116 promotes the maturation of cardiomyocytes derived from human pluripotent stem cells.MOLECULES AND CELLS,45(12),869~975.
MLA	Ji, Sijia,et al."The Aurora kinase inhibitor CYC116 promotes the maturation of cardiomyocytes derived from human pluripotent stem cells".MOLECULES AND CELLS 45.12(2022):869~975.