Cis- and trans-regulation by histone H4 basic patch R17/R19 in metazoan development

doi:10.1098/rsob.220066

	Cis- and trans-regulation by histone H4 basic patch R17/R19 in metazoan development
	Zhang, Xuedi; Wu, Xiangyu; Peng, Ju; Sun, Angyang; Guo, Yan; Fu, Pengchong; Gao, Guanjun
	2022-11-16
发表期刊	OPEN BIOLOGY (IF:4.5[JCR-2023],5.9[5-Year])
EISSN	2046-2441
卷号	12 期号:11
发表状态	已发表
DOI	10.1098/rsob.220066
摘要	The histone H4 basic patch is critical for chromatin structure and regulation of the chromatin machinery. However, the biological roles of these positively charged residues and the mechanisms by which they regulate gene expression remain unclear. In this study, we used histone mutagenesis to investigate the physiological function and downstream regulatory genes of H4 residues R17 and R19 in Drosophila. We found all histone mutations including R17A/E/H and R19A/E/H (R17 and R19 of H4 are substituted by A, E and H respectively) result in a range of growth defects and abnormalities in chromosomal high-order structures, whereas R17E mutation is embryonic lethal. RNA-seq demonstrates that downregulated genes in both R17A and R19A show significant overlap and are enriched in development-related pathways. In addition, Western and cytological analyses showed that the R17A mutation resulted in a significant reduction in H4K16 acetylation and male offspring, implying that the R17 may be involved in male dosage compensation mechanisms. R19 mutation on the other hand strongly affect Gpp (Dot1 homologue in flies)-mediated H3K79 methylation, possibly through histone crosstalk. Together these results provide insights into the differential impacts of positive charges of H4 basic patch R17/R19 on regulation of gene transcription during developmental processes.
关键词	Drosophila development histone H4 basic patch
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收录类别	SCI ; SCIE
语种	英语
资助项目	National Postdoctoral Fellowship["32070846","91740107"] ; Science and Technology Commission of Shanghai Municipality[2019M661652] ; [19JC1413600]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000885610700001
出版者	ROYAL SOC
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/251701
专题	生命科学与技术学院_PI研究组_高冠军组生命科学与技术学院_硕士生生命科学与技术学院_博士生
通讯作者	Gao, Guanjun
作者单位	ShanghaiTech Univ, Sch Life Sci & Technol, 393 Middle Huaxia Rd, Pudong 201210, Shanghai, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhang, Xuedi,Wu, Xiangyu,Peng, Ju,et al. Cis- and trans-regulation by histone H4 basic patch R17/R19 in metazoan development[J]. OPEN BIOLOGY,2022,12(11).
APA	Zhang, Xuedi.,Wu, Xiangyu.,Peng, Ju.,Sun, Angyang.,Guo, Yan.,...&Gao, Guanjun.(2022).Cis- and trans-regulation by histone H4 basic patch R17/R19 in metazoan development.OPEN BIOLOGY,12(11).
MLA	Zhang, Xuedi,et al."Cis- and trans-regulation by histone H4 basic patch R17/R19 in metazoan development".OPEN BIOLOGY 12.11(2022).